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Drug Res (Stuttg) 2015; 65(11): 614-616
DOI: 10.1055/s-0034-1395628
Short Communication
© Georg Thieme Verlag KG Stuttgart · New York

A Supramolecular Substance, [2] Rotaxane, Induces Apoptosis in Human Molt-3 Acute Lymphoblastic Leukemia Cells

M. Kimura
1   Medicinal-Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan
,
K. Makio
1   Medicinal-Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan
4   Fukuoka Prefecture South Chikugo Health and Welfare Environment Office, Yanagawa, Japan
,
K. Hara
1   Medicinal-Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan
,
W. Hiruma
1   Medicinal-Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan
,
Y. Fujita
3   Department of Genome Biology, Kinki University School of Medicine, Osaka, Japan
,
T. Takata
2   Department of Organic and Polymeric Materials, Tokyo Institute of Technology, Tokyo, Japan
,
K. Nishio
3   Department of Genome Biology, Kinki University School of Medicine, Osaka, Japan
,
N. Ono
1   Medicinal-Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan
› Author Affiliations
Further Information

Publication History

received 12 October 2013

accepted 04 November 2014

Publication Date:
02 December 2014 (online)

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Abstract

The antitumor effects of a supramolecular substance, the [2] rotaxane (TRO-A0001), and its molecular mechanisms were investigated. TRO-A0001 suppressed the proliferation of cultured human Molt-3 acute lymphoblastic leukemia cells for 12–72 h in a dose-dependent manner. Based on flow cytometry, TRO-A0001 clearly induced apoptosis after 24 h. The mitochondrial membrane potential disappeared after treatment with 1.0 µM of TRO-A0001. Expression of the cleaved forms of capase-9 and caspase-3 was significantly increased in cells exposed to TRO-A0001, whereas the expression of XIAP, a type of inhibitor of apoptosis family, was decreased. These results suggest that [2] rotaxane TRO-A0001 may be a highly promising new antitumor medicine.


 

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