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Planta Med 2014; 80(13): 1143-1150
DOI: 10.1055/s-0034-1382959
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Establishment of an Enzyme-Linked Immunosorbent Assay and Application on Determination of Ginsenoside Re in Human Saliva

Huihua Qu   *
1   Center of Scientific Experiment, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Jiayang Sai   *
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Yan Wang
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Ye Sun
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Yue Zhang
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Yifei Li
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Yan Zhao
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
,
Qingguo Wang
2   School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China
› Author Affiliations
Further Information

Publication History

received 03 December 2013
revised 10 July 2014

accepted 10 July 2014

Publication Date:
08 September 2014 (online)

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Abstract

This work describes an immunochemical approach for the quality control of Panax ginseng and a pharmacological study of ginsenoside Re, a major bioactive constituent in P. ginseng, using an enzyme-linked immunosorbent assay. A hybridoma secreting monoclonal antibody against ginsenoside Re was produced by fusing splenocytes immunized with a ginsenoside Re-bovine serum albumin conjugate with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma SP2/0 cell line. The method, at an effective measuring range of 7.8–500 ng · mL−1 of ginsenoside Re, successfully detected ginsenoside Re in Chinese traditional herb prescriptions. The results demonstrate that we generated a novel and reliable assay system for measuring ginsenoside Re in Chinese medicines more efficiently. Futhermore, we determined the ginsenoside Re concentrations in the saliva of six healthy adults after the oral administration of a ginseng capsule to study the pharmacokinetics of ginsenoside Re in human saliva.

Key words

ginsenoside Re - ginsenoside Rg1 - monoclonal antibody - enzyme-linked immunosorbent assay - human saliva

* These two authors contributed equally to this work.


 

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