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Planta Med 2014; 80(06): 517-522
DOI: 10.1055/s-0034-1368348
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Simultaneous Determination of Eight Active Components in the Traditional Chinese Medicine Dan Deng Tong Nao Capsules by HPLC-MS/MS

Baohua Wu
Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, Hangzhou, Peopleʼs Republic of China
,
Xingguo Zhang
Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, Hangzhou, Peopleʼs Republic of China
,
Dongsheng Hong
Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, Hangzhou, Peopleʼs Republic of China
,
Kuifen Ma
Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, Hangzhou, Peopleʼs Republic of China
,
Saiping Jiang
Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, Hangzhou, Peopleʼs Republic of China
› Author Affiliations
Further Information

Publication History

received 05 July 2013
revised 14 February 2014

accepted 02 March 2014

Publication Date:
07 April 2014 (online)

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Abstract

An high-performance liquid chromatography-tandem mass spectrometry method has been optimised and established for the quality evaluation of the traditional Chinese medicine Dan Deng Tong Nao capsules through simultaneous determination of the following eight active components: danshensu, salvianolic acid A, salvianolic acid C, puerarin, scutellarin, apigenin, 3,4-dihydroxybenzaldehyde, and ferulic acid. All of the analytes were separated on a Waters Xbridge™ C18 column (4.6 × 150 mm, 3.5 µm particle size) with a mobile phase consisting of methanol/acetonitrile (50 : 50, v/v) and water containing 0.1 % formic acid. All of the compounds showed good linearity (R2 > 0.997). The recoveries, measured at three concentration levels, varied from 94.94 to 107.3 %. The validated method was successfully applied to evaluate the eight active components in Dan Deng Tong Nao capsules collected from different production batches. The results suggested that the method established in this study could be considered a good approach to controlling the quality of Dan Deng Tong Nao capsules and other related botanical drugs.

Key words

Dan Deng Tong Nao capsules - quantitative analysis - quality evaluation - LC-MS/MS
 

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