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Planta Med 2014; 80(06): 490-492
DOI: 10.1055/s-0034-1368298
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

Comparative Evaluation of Antiproliferative Effects of Brazilian Green Propolis, Its Main Source Baccharis dracunculifolia, and Their Major Constituents Artepillin C and Baccharin

Pollyanna Francielli de Oliveira
1   Universidade de Franca, Franca, São Paulo, Brazil
,
Ildercílio Mota de Souza Lima
1   Universidade de Franca, Franca, São Paulo, Brazil
,
Carla Carolina Munari
1   Universidade de Franca, Franca, São Paulo, Brazil
,
Jairo Kenupp Bastos
2   Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil
,
Ademar Alves da Silva Filho
3   Faculdade de Farmácia, Departamento de Ciências Farmacêuticas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil
,
Denise Crispim Tavares
1   Universidade de Franca, Franca, São Paulo, Brazil
› Author Affiliations
Further Information

Publication History

received 29 November 2013
revised 20 February 2014

accepted 24 February 2014

Publication Date:
31 March 2014 (online)

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Abstract

This study evaluated the antiproliferative activity of the Brazilian green propolis and Baccharis dracunculifolia extracts and their major compounds artepillin C and baccharin in different tumor cell lines. The lowest IC50 values observed for Brazilian green propolis and B. dracunculifolia extracts were 41.0 ± 4.5 µg/mL for U343 and 44.9 ± 7.1 µg/mL for HepG2, respectively. Regarding artepillin C and baccharin, the lowest IC50 values were 20.1 ± 2.9 for U343 and 13.0 ± 1.5 µg/mL for B16F10, respectively. For the association of artepillin C plus baccharin, the lowest IC50 result was 35.2 ± 0.5 µg/mL for B16F10. Artepillin C and baccharin were more cytotoxic than both Brazilian green propolis and B. dracunculifolia extracts. No additive or synergistic effect was observed for the association of artepillin C plus baccharin.

Key words

green propolis - Baccharis dracunculifolia - Asteraceae - artepillin C - baccharin

Supporting Information

 

  • References

  • 1 Marcucci MC, Ferreres F, García-Viguera C, Bankova VS, de Castro SL, Dantas AP, Valente PHM, Paulino N. Phenolic compounds from Brazilian propolis with pharmacological activities. J Ethnopharmacol 2001; 74: 105-112
    CrossrefPubMedGoogle Scholar
  • 2 Sousa JP, Bueno PC, Gregório LE, da Silva Filho AA, Furtado NA, de Sousa ML, Bastos JK. A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography. J Sep Sci 2007; 30: 2656-2665
    CrossrefPubMedGoogle Scholar
  • 3 Da Silva Filho AA, de Sousa JPB, Soares S, Furtado NAJC, Andrade e Silva ML, Cunha WR, Gregório LE, Nanayakkara NPD, Bastos JK. Antimicrobial activity of the extract and isolated compounds from Baccharis dracunculifolia D.C. (Asteraceae). Z Naturforsch 2008; 63: 40-46
    PubMedGoogle Scholar
  • 4 Hernandez J, Goycoolea FM, Quintero J, Acosta A, Castañeda M, Dominguez Z, Robles R, Vazquez-Moreno L, Velazquez EF, Astiazaran H, Lugo E, Velazquez C. Sonoran propolis: chemical composition and antiproliferative activity on cancer cell lines. Planta Med 2007; 73: 1469-1474
    Article in Thieme ConnectPubMedGoogle Scholar
  • 5 Chanchao C, Umthong S, Phuwapraisirisan P, Puthong S. Trigona laeviceps propolis: Chemical composition and antiproliferative activity on cancer cell lines. Planta Med 2011; 77: PM49 DOI: 10.1055/s-0031-1282807.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 6 Munari CC, Resende FA, Alves JM, Sousa JP, Bastos JK, Tavares DC. Mutagenicity and antimutagenicity of Baccharis dracunculifolia extract in chromosomal aberration assays in Chinese hamster ovary cells. Planta Med 2008; 74: 1363-1367
    Article in Thieme ConnectPubMedGoogle Scholar
  • 7 Tavares DC, Lira WM, Santini CB, Takahashi CS, Bastos JK. Effects of propolis crude hydroalcoholic extract on chromosomal aberrations induced by doxorubicin in rats. Planta Med 2007; 73: 1531-1546
    Article in Thieme ConnectPubMedGoogle Scholar
  • 8 Jin UH, Chung TW, Kang SK, Suh SJ, Kim JK, Chung KH, Gu YH, Suzuki I, Kim CH. Caffeic acid phenyl ester in propolis is a strong inhibitor of matrix metalloproteinase-9 and invasion inhibitor: isolation and identification. Clin Chim Acta 2005; 362: 57-64
    CrossrefPubMedGoogle Scholar
  • 9 Matsuno T, Jung SK, Matsumoto Y, Saito M, Morikawa J. Preferential cytotoxicity to tumor cells of 3, 5-diprenyl-4-hydroxycinnamicacid (artepillin C) isolated from propolis. Anticancer Res 1997; 17: 3565-3568
    PubMedGoogle Scholar
  • 10 Kimoto T, Aga M, Hino K, Koya-Miyata S, Yamamoto Y, Micallef MJ, Hanaya T, Arai S, Ikeda M, Kurimoto M. Apoptosis of human leukemia cells induced by artepillin C, an active ingredient of Brazilian propolis. Anticancer Res 2001; 21: 221-228
    PubMedGoogle Scholar
  • 11 Szliszka E, Zydowicz G, Mizgala E, Krol W. Artepillin C (3, 5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis. Int J Oncol 2012; 41: 818-828
    CrossrefPubMedGoogle Scholar
  • 12 Akao Y, Maruyama H, Matsumoto K, Ohguchi K, Nishizawa K, Sakamoto T, Araki Y, Mishima S, Nozawa Y. Cell growth inhibitory effect of cinnamic acid derivatives from propolis on human tumor cell lines. Biol Pharm Bull 2003; 26: 1057-1059
    CrossrefPubMedGoogle Scholar
  • 13 Endo S, Matsunaga T, Kanamori A, Otsuji Y, Nagai H, Sundaram K, El-Kabbani O, Toyooka N, Ohta S, Hara A. Selective inhibition of human type-5 17β-hydroxysteroid dehydrogenase (AKR1C3) by baccharin, a component of Brazilian propolis. J Nat Prod 2012; 75: 716-721
    CrossrefPubMedGoogle Scholar
  • 14 De P, Baltas M, Bedos-Belval F. Cinnamic acid derivatives as anticancer agents – a review. Curr Med Chem 2011; 18: 1672-1703
    CrossrefPubMedGoogle Scholar
  • 15 Mishima S, Ono Y, Araki Y, Nozawa Y. Two related cinnamic acid derivatives from Brazilian honey propolis, baccharin and drupanin, induce growth inhibition in allografted sarcoma S-180 in mice. Biol Pharm Bull 2005; 28: 1025-1030
    CrossrefPubMedGoogle Scholar
  • 16 Simões LM, Gregorio LE, da Silva Filho AA, de Souza ML, Azzolini AECS, Bastos JK, Lucisano-Valim YM. Effect of Brazilian green propolis on the production of reactive oxygen species by stimulated neutrophils. J Ethnopharmacol 2004; 94: 59-65
    CrossrefPubMedGoogle Scholar
  • 17 Da Silva Filho AA, Pires Bueno PC, Gregório LE, Andrade e Silva ML, Albuquerque S, Bastos JK. In vitro trypanocidal evaluation of crude extract and isolated compounds from Baccharis dracunculifolia D.C. (Asteraceae). J Pharm Pharmacol 2004; 56: 1195-1199
    CrossrefPubMedGoogle Scholar
  • 18 Sousa JPB, Da Silva Filho AA, Bueno PCP, Gregório LE, Furtado NAJC, Bastos JK. A validated reverse phase HPLC analytical method for the quantification of phenolic compounds in Baccharis dracunculifolia . Phytochem Anal 2009; 20: 24-32
    CrossrefPubMedGoogle Scholar
  • 19 Shimizu K, Das SK, Baba M, Matsuura Y, Kanazawa K. Dietary artepillin C suppresses the formation of aberrant crypt foci induced by azoxymethane in mouse colon. Cancer Lett 2006; 240: 135-142
    CrossrefPubMedGoogle Scholar
  • 20 Sousa JPB, Furtado NAJC, Jorge R, Soares AEE, Bastos JK. Perfis físico-químicos e cromatográficos de amostras de própolis produzidas nas microrregiões de Franca (SP) e Passos (MG), Brasil. Rev Bras Farmacog 2007; 17: 85-93
    CrossrefPubMedGoogle Scholar