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Planta Med 2014; 80(01): 56-62
DOI: 10.1055/s-0033-1360174
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

Ivan Čavarga
1   Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia
2   St Elizabeth Oncological Institute, Bratislava, Slovakia
,
Boris Bilčík
1   Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia
,
Pavel Výboh
1   Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia
,
Monika Záškvarová
1   Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia
,
Dušan Chorvát
3   International Laser Centre, Bratislava, Slovakia
,
Peter Kasák
4   Polymer Institute, Slovak Academy of Sciences, Bratislava, Slovakia
,
Peter Mlkvý
2   St Elizabeth Oncological Institute, Bratislava, Slovakia
,
Anton Mateašík
3   International Laser Centre, Bratislava, Slovakia
,
Alžbeta Chorvátová
3   International Laser Centre, Bratislava, Slovakia
,
Pavol Miškovský
5   Dept. of Biophysics, Faculty of Sciences, P. J. Šafárik University, Košice, Slovakia
6   Center for Interdisciplinary Biosciences, P. J. Šafárik University, Košice, Slovakia
› Author Affiliations
Further Information

Publication History

received 07 August 2013
revised 18 October 2013

accepted 19 November 2013

Publication Date:
10 January 2014 (online)

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Abstract

Photosensitizing properties of hypericin are well known, and the chicken chorioallantoic membrane has previously been used to test photodynamic effects of hypericin and other substances. In our study the photodynamic effect of hypericin in the ex ovo quail chorioallantoic membrane model was evaluated. Steady-state and time-resolved fluorescence spectroscopy of hypericin solution in PEG-400 and its mixture in PBS was performed to assess and characterize the process of aggregation and disaggregation of hypericin during the drug formulation preparation. A therapeutical formulation (2 µg/g of embryo weight) was topically applied on CAM into the silicone ring. Hypericin was excited by diode laser with wavelength 405 nm, fluence rate 140 mW/cm2, and fluence 16.8 J/cm2. Hypericin in 100 % PEG-400 exhibits typical fluorescence spectra with a maximum of about 600 nm, while hypericin 10% PEG-400 formulation exhibits almost no fluorescence. Time resolved spectra analysis showed fluorescence decay of hypericin in 100 % PEG-400 solution with a mean lifetime of 5.1 ns and in 10 % PEG 4.1 ns. Damage of quail chorioallantoic membrane vasculature after photodynamic therapy ranged from hemorrhage and vanishing of capillary vessels to thrombosis, lysis, and hemorrhage of larger vessels.

The presented findings suggest that quail embryos can be used as a suitable model to test the effect of hypericin and other photodynamic compounds.

Key words

hypericin - Japanese quail - chorioallantoic membrane - photodynamic therapy - aggregation - Hypericum - Hypericaceae
 

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