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Int J Sports Med 2012; 33(11): 886-891
DOI: 10.1055/s-0032-1301887
Training & Testing
© Georg Thieme Verlag KG Stuttgart · New York

High Intensity Exercise Affects Diurnal Variation of Some Biological Markers in Trained Subjects

O. Hammouda
1   Research Laboratory “Sports performance optimization” National Center of Medicine and Science in Sports (CNMSS), Tunis, Tunisia
2   Research Unit, High Institute of Sport and Physical Education, Sfax, Tunisia
,
H. Chtourou
1   Research Laboratory “Sports performance optimization” National Center of Medicine and Science in Sports (CNMSS), Tunis, Tunisia
,
H. Chahed
3   Laboratory of biochemistry CHU Farhat Hached, Sousse, Tunisia
,
S. Ferchichi
3   Laboratory of biochemistry CHU Farhat Hached, Sousse, Tunisia
,
A. Chaouachi
1   Research Laboratory “Sports performance optimization” National Center of Medicine and Science in Sports (CNMSS), Tunis, Tunisia
,
C. Kallel
4   Laboratory of hematology Habib Bourguiba hospital, Sfax, Tunisia
,
A. Miled
3   Laboratory of biochemistry CHU Farhat Hached, Sousse, Tunisia
,
K. Chamari
1   Research Laboratory “Sports performance optimization” National Center of Medicine and Science in Sports (CNMSS), Tunis, Tunisia
5   High Institute of Sport and Physical Education, Ksar-Saïd, Manouba University, Tunisia
,
N. Souissi
1   Research Laboratory “Sports performance optimization” National Center of Medicine and Science in Sports (CNMSS), Tunis, Tunisia
5   High Institute of Sport and Physical Education, Ksar-Saïd, Manouba University, Tunisia
› Author Affiliations
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Publication History



accepted after revision 21 December 2011

Publication Date:
12 July 2012 (online)

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Abstract

The study investigated if markers of muscle injury and antioxidant status were affected by a Wingate test performed at 2 different times of day. 15 young male footballers performed 2 tests (randomized) at 07:00-h and 17:00-h. Fasting blood samples were collected before and 3 min after each test for assessment of markers of muscle injury and antioxidant status. Resting oral temperature was recorded during each session. Peak power (10.76±1.05 vs. 11.15±0.83 W.kg − 1) and fatigue index (0.41±0.04 vs. 0.49±0.13%) during the Wingate test, and core temperature, were significantly higher (all p<0.05) in the evening. Markers of muscle injury were significantly higher in the evening before and after exercise (e. g., 148.7±67.05 vs. 195±74.6 and 191.6±79.52 vs. 263.6±96.06 IU.L − 1, respectively, for creatine kinase; both p<0.001). Antioxidant parameters increased after the Wingate test but only resting values were significantly higher in the morning (e. g., 1.33±0.19 vs. 1.19±0.14 µmol.L − 1 for total antioxidant status; p<0.05). The results indicate that muscle injury and antioxidant activity after the Wingate test were higher in the evening, suggesting a possible link between the biochemical measures and the diurnal fluctuation of anaerobic performance. However, repetition of this study after prescribed rather than self-selected exercise intensity is recommended.

Key words

time of day - muscle injury - anaerobic exercise - leucocytes - total antioxidant status
 

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