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Horm Metab Res 2009; 41(11): 805-813
DOI: 10.1055/s-0029-1225631
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Transcripts of Calcium/Calmodulin-dependent Kinases are Changed After Forskolin- or IBMX-induced Insulin Secretion due to Melatonin Treatment of Rat Insulinoma β-Cells (INS-1)[*]

I. Bazwinsky-Wutschke1 , E. Mühlbauer2 , S. Wolgast2 , E. Peschke1
  • 1Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle, Germany
  • 2Saxon Academy of Sciences Leipzig, Leipzig, Germany
Further Information

Publication History

received 18.03.2009

accepted 04.06.2009

Publication Date:
13 July 2009 (online)

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Abstract

The objective of the present study was to examine the effects of melatonin on transcripts of isoforms of calcium/calmodulin-dependent protein kinases in rat insulinoma β-cells INS-1. Investigations show that calcium/calmodulin-dependent kinase IV and calcium/calmodulin-dependent kinase 2d are expressed in human and rat pancreatic islets and INS-1 cells. By application of either forskolin or 3-isobutyl-1-methylxanthine for 6 hours, calcium spiking was evoked and the release of insulin was increased. The expression of the calcium/calmodulin-dependent kinase IV and calcium/calmodulin-dependent kinase 2d transcripts was significantly increased due to forskolin or 3-isobutyl-1-methylxanthine. Acute melatonin treatment (6 h) in the presence of either forskolin or 3-isobutyl-1-methylxanthine caused a significant decrease in insulin release and induced significant downregulation of calcium/calmodulin-dependent kinase IV and calcium/calmodulin-dependent kinase 2d transcripts in INS-1 batch cultures. The attenuating effect of melatonin on transcripts could be almost completely reversed by preincubation with the melatonin receptor antagonist luzindole. Thus, the insulin-inhibiting effect of melatonin in INS-1 cells is associated with significant changes in transcripts of calcium-signaling components suggesting that melatonin influences gene expression of components, which are known to be involved in insulin secretion or insulin gene expression.

Key words

calcium/calmodulin-dependent kinase IV - calcium/calmodulin-dependent kinase 2d - pancreatic islet - insulin secretion

1 Dedicated to Professor Dr. Dr. Bernd Fischer on the occasion of his 60th birthday, August 25th 2009.

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1 Dedicated to Professor Dr. Dr. Bernd Fischer on the occasion of his 60th birthday, August 25th 2009.

Correspondence

Dr. I. Bazwinsky-Wutschke

Institute of Anatomy and Cell Biology

Martin Luther University Halle-Wittenberg

Grosse Steinstrasse 52

06097 Halle

Germany

Phone: +49/34/5557 17 10

Fax: +49/34/5557 40 53

Email: ivonne.bazwinsky@medizin.uni-halle.de

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