STOFFWECHSEL UND ABBAU VON NICOTINSÄURE UND IHREN DERIVATEN IN PFLANZLICHEN ZELLSUSPENSIONSKULTUREN

Abstract

The uptake of nicotinic acid, nicotinamide, trigonellin and NAD by cell suspension cultures of mung bean, soybean, garbanzo bean and parsley decreased with increasing charge of substrates. The legumenous cell cultures convert nicotinic acid mainly to trigonellin whereas the parsley cultures form nicotinic acid–N–arabinoside. Furthermore only the parsley cell cultures can decarboxylate nicotinic acid, nicotinamide and NAD, respectively, as well as catalyze ring fission reactions of nicotinic acid.

All four cell cultures showed no decarboxylation of trigonellin. Mung bean and garbanzo bean converted the methyl group of trigonellin mainly to CO ₂ though transmethylation reactions could also be observed.