Modulation of Macrophage Functions by Compounds Isolated from Zingiber officinale

Abstract

Bioactivity-guided fractionation of Zingiber officinale (Zingiberaceae) led us to isolate 14 compounds, [4]-gingerol (1), [6]-gingerol (2), [8]-gingerol (3), [10]-gingerol (4), [6]-paradol (5), [4]-shogaol (6), [6]-shogaol (7), 1-dehydro-[10]-gingerdione (8), [10]-gingerdione (9), hexahydrocurcumin (10), tetrahydrocurcumin (11), gingerenone A (12), 1,7-bis-(4’ hydroxyl-3’ methoxyphenyl)-5-methoxyhepthan-3-one (13), and methoxy-[10]-gingerol (14). Using the RAW 264.7 cell line, the inhibitory effects on nitric oxide production induced by lipopolysaccharide and the stimulatory effects on phagocytosis of these compounds were evaluated. Compounds 7, 8, and 9 significantly decreased lipopolysaccharide-induced nitric oxide production, and compounds 7 and 8 significantly reduced inducible nitric oxide synthase expression. Among them, compound 8 also showed significant stimulatory effects on phagocytosis.

Abbreviations

Con A:concanavalin A

DMEM:Dulbecco’s modified Eagle’s medium

FBS:fetal bovine serum

iNOS:inducible nitric oxide synthase

LPS:lipopolysaccharide

NO:nitric oxide

TTBS:Tween tris buffered saline

Key words

Zingiber officinale - Zingiberaceae - macrophage - nitric oxide - phagocytic activity

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