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Planta Med 2022; 88(12): 964-974
DOI: 10.1055/a-1535-2230
Natural Product Chemistry and Analytical Studies
Original Papers

The Standardized Wood Extract of Albizia myriophylla: Its Potential as an Active Ingredient in an Anti-inflammatory Herbal Gel Formulation

Nazneen Bakasatae
1   Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla, Thailand
,
Warissara Sangkaew
1   Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla, Thailand
,
Nongluk Kunworarath
1   Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla, Thailand
,
Julalak Chorachoo Ontong
2   Cosmetic Technology and Dietary Supplement Products Program, Faculty of Agro and Bio Industry, Thaksin University, Phatthalung, Thailand
,
Sathianpong Phoopha
1   Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla, Thailand
,
Chutha Takahashi Yupanqui
3   Interdisciplinary Graduate School of Nutraceutical and Functional Food, Prince of Songkla University, Songkhla, Thailand
,
Supayang Piyawan Voravuthikunchai
4   Excellent Research Laboratory on Natural Products, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Songkhla, Thailand
5   Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla, Thailand
,
Nantiya Joycharat
1   Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla, Thailand
4   Excellent Research Laboratory on Natural Products, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Songkhla, Thailand
› Author AffiliationsSupported by: TRF Senior Research Scholar RTA6180006
Supported by: the annual income budget of Prince of Songkla University TTM600563S and TTM6302212S
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Abstract

Albizia myriophylla has been used in Thai folk medicine for treating inflammation-related diseases. The wood of this medicinal plant is traditionally used as a single herbal drug in the form of an aqueous decoction and as a component in several Thai herbal formulations for the remedy of fever, sore throat, and aphthous ulcers. This study aimed to evaluate in vivo the anti-inflammatory potential and possible mechanism of action of the standardized wood extract of A. myriophylla as well as to investigate the anti-inflammatory activity and physicochemical properties of the developed herbal gel formulation containing standardized wood extract of A. myriophylla. Results of quantitative HPLC analysis demonstrated that the standardized wood extract of A. myriophylla contained 22.95 mg/g of 8-methoxy-7,3′,4′-trihydroxyflavone, a bioactive marker compound of A. myriophylla. The standardized wood extract of A. myriophylla (1% w/v) exhibited remarkable inhibition (54.4 – 80.3%) in the croton oil model of topical inflammation at all assessment times, comparable to standard indomethacin (55.3 – 63.6%). Real-time quantitative reverse transcription-polymerase chain reaction was performed to clarify the anti-inflammatory mechanism of standardized wood extract of A. myriophylla, and the result showed that this standardized extract decreased lipopolysaccharide-induced nitric oxide synthase mRNA levels in a dose-dependent manner. The developed herbal gel containing standardized wood extract of A. myriophylla (1% w/w) had good physicochemical characteristics and exhibited potent inhibition (51.4 – 77.8%) of inflammation in a rat ear edema model at all assessment times, comparable to indomethacin gel (33.3 – 40.5%). The notable anti-inflammatory activity of standardized wood extract of A. myriophylla and its developed herbal gel formulation indicates their potential application as natural anti-inflammatory agents.

Key words

Albizia myriophylla - Fabaceae - herbal gel formulation - inflammation - standardized extract

Supporting Information

    Chemical profiles in the fingerprints of the Std-AM and the herbal gel formulation containing 1% Std-AM (Table 1S) as well as the data leading to IC50 determinations as dose-response curves of herbal gel, indomethacin gel, gel base, and standard (Fig. 1S) are available as Supporting Information.

  • Supporting Information


Publication History

Received: 01 April 2021

Accepted after revision: 21 June 2021

Article published online:
06 August 2021

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