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Planta Med 2021; 87(05): 368-374
DOI: 10.1055/a-1266-6674
Biological and Pharmacological Activity
Original Papers

Achiote (Bixa orellana) Lipophilic Extract, Bixin, and δ-tocotrienol Effects on Lifespan and Stress Resistance in Caenorhabditis elegans

Darío R. Gómez-Linton
1   Programa de Doctorado en Biotecnología, Universidad Autónoma Metropolitana-I, Iztapalapa, Ciudad de México, México
,
Silvestre Alavez
2   Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-L, Lerma de Villada, Estado de México, México
,
Arturo Navarro-Ocaña
3   Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México
,
Angélica Román-Guerrero
4   Departamento de Biotecnología, Universidad Autónoma Metropolitana-I, Iztapalapa, Ciudad de México, México
,
Luis Pinzón-López
5   Instituto Tecnológico de Conkal, Yucatán, México
,
Laura J. Pérez-Flores
6   Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-I, Iztapalapa, Ciudad de México, México
› Author AffiliationsSupported by: Consejo Nacional de Ciencia y Tecnología 300612
Supported by: Consejo Nacional de Ciencia y Tecnología 518007
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Abstract

The onset of many degenerative diseases related to aging has been associated with a decrease in the activity of antistress systems, and pharmacological interventions increasing stress resistance could be effective to prevent the development of such diseases. Achiote is a valuable source of carotenoid and tocotrienols, which have antioxidant activity. In this work, we explore the capacity of an achiote seed extract and its main compounds to modulate the lifespan and antistress responses on Caenorhabditis elegans, as well as the mechanisms involved in these effects. Achiote lipophilic extract, bixin, and δ-tocotrienol were applied on nematodes to carry out lifespan, stress resistance, and fertility assays. The achiote seed extract increased the median and maximum lifespan up to 35% and 27% and increased resistance against oxidative and thermal stresses without adverse effects on fertility. The beneficial effects were mimicked by a bixin+δ-tocotrienol mixture. All the effects on lifespan and stress resistance were independent of caloric restriction but dependent on the insulin/insulin growth factor-1 pathway. This study could provide insights for further research on a new beneficial use of this important crop in health and nutraceutical applications beyond its use as a source of natural pigments.

Key words

Bixaceae - Bixa Orellana - Caenorhabditis elegans - antioxidants - longevity - stress resistance

Supporting Information

    Representative chromatograms of bixin (Fig. 1S) and tocochromanol (Fig. 2S) determinations are presented in the Supporting Information. The effect of 3 concentrations of AE (Fig. 3S) and of bixin, dT3, and the mixture thereof (Fig. 4S) on the resistance against UV radiation is presented in the Supporting Information.

  • Supporting Information


Publication History

Received: 07 April 2020

Accepted after revision: 14 September 2020

Article published online:
29 October 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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