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Planta Med 2020; 86(15): 1148-1155
DOI: 10.1055/a-1176-1937
Natural Product Chemistry and Analytical Studies
Original Papers

Differentiation between Cistus L. (Sub-) Species (Cistaceae) Using NMR Metabolic Fingerprinting[#]

Simon Moosmang
1   University of Innsbruck, Institute for Pharmacy/Department of Pharmacognosy, Center for Molecular Biosciences Innsbruck (CMBI), Innsbruck, Austria
,
Sonja Sturm
1   University of Innsbruck, Institute for Pharmacy/Department of Pharmacognosy, Center for Molecular Biosciences Innsbruck (CMBI), Innsbruck, Austria
,
Johannes Novak
2   University of Veterinary Medicine Vienna, Institute for Animal Nutrition and Functional Plant Compounds, Vienna, Austria
,
Brigitte Lukas
2   University of Veterinary Medicine Vienna, Institute for Animal Nutrition and Functional Plant Compounds, Vienna, Austria
,
Hermann Stuppner
1   University of Innsbruck, Institute for Pharmacy/Department of Pharmacognosy, Center for Molecular Biosciences Innsbruck (CMBI), Innsbruck, Austria
› Author AffiliationsSupported by: Austrian Science Fund P 29305
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Abstract

The genus Cistus is taxonomically complex, as taxonomic classification of individual species based on morphological criteria is often difficult and ambiguous. However, specific species contain valuable natural products, especially terpenoids and polyphenols, which exert various biological effects and might therefore be used for treatment of a broad array of disorders. Hence, a fast and reliable method for clear identification of different Cistus (sub-) species is required. Approaches for analysis of secondary metabolite profiles, e.g., with NMR, might remedy the challenging classification of Cistus (sub-) species and help to identify specific markers for differentiation between them. In the present study, 678 samples from wild-growing Cistus populations, including 7 species and 6 subspecies/varieties thereof, were collected in 3 years from populations in 11 countries all over the Mediterranean basin. Samples were extracted with buffered aqueous methanol and analysed with NMR. From the resulting 1D-1H-NOESY and J-Res profile spectra, marker signals or spectral regions for the individual (sub-) species were identified with multivariate statistical tools. By examining the NMR profiles of these extracts, we were able to identify discriminators and specific markers for the investigated Cistus (sub-) species. Various influencing factors, like (sub-) species, wild harvestings of different populations from several countries, numerous collection sites, different years, and cultivation in greenhouses have been considered in this work. As the here identified markers are independent from these influencing factors, the results can be considered a robust model and might be used for future differentiation between Cistus (sub-) species.

Key words

Cistus L. - Cistaceae - NMR-secondary metabolites - metabolic fingerprinting - chemotaxonomic classification

# Dedicated to Prof. Wolfgang Kubelka on the occasion of his 85th birthday.


Supporting Information

    Information of NMR pulse programs, country-specific floras used for taxonomic classification, a breakdown of sample numbers by country and species, PCA and OPLS-DA scores and loadings plots, results of permutation tests used for model validation, boxplots comparing signal intensities between different Cistus species, and plots with mean bucket integrals of different Cistus (sub-) species are available as Supporting Information.

  • Supporting Information


Publication History

Received: 10 January 2020

Accepted after revision: 05 May 2020

Article published online:
03 June 2020

© 2020. Thieme. All rights reserved.

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