Download PDF
Homeopathy 2009; 98(02): 92-96
DOI: 10.1016/j.homp.2009.02.010
Original Paper
Copyright © The Faculty of Homeopathy 2009

Homeopathic treatment for bone regeneration: experimental study

Janete Dias Almeida
1   Department of Bioscience and Oral Diagnosis, São Paulo State University – UNESP, São José dos Campos Dental School, São José dos Campos, São Paulo, Brazil
,
Emília Angela Loschiavo Arisawa
2   Department of Pathology, UNIVAP, Vale do Paraíba University, Health Science Faculty, São José dos Campos, São Paulo, Brazil
,
Ivan Balducci
3   Department of Social Science and Pediatric Dentistry, São Paulo State University – UNESP, São José dos Campos Dental School, São José dos Campos, São Paulo, Brazil
,
Rosilene Fernandes da Rocha
1   Department of Bioscience and Oral Diagnosis, São Paulo State University – UNESP, São José dos Campos Dental School, São José dos Campos, São Paulo, Brazil
,
Yasmin Rodarte Carvalho
1   Department of Bioscience and Oral Diagnosis, São Paulo State University – UNESP, São José dos Campos Dental School, São José dos Campos, São Paulo, Brazil
› Author Affiliations
Subject Editor:
Further Information

Publication History

Received07 December 2007
revised13 August 2008

accepted04 February 2009

Publication Date:
20 December 2017 (online)

    Permissions and Reprints

Aim and method: The objective of this research was to study the effect of homeopathic treatment with Plumbum metallicum (Plumbum met.) on mandibular bone repair in rats.

Materials and methods: We analyzed the mandibles of 60 male rats, approximately 3-month-old, randomly divided into three groups of 20 animals each: control, treated with calcitonin, and treated with a homeopathic medicine. A circumscribed bone defect measuring 4 mm in diameter was made in the mandible and covered with a polytetrafluorethylene (PTFE) barrier. The group treated with calcitonin received 2 IU/kg intramuscularly three times a week; the group treated with Plumbum met. 30c received three drops in water every day. The animals were sacrificed after 7, 14, 21 and 28 days. The mandibles were removed and submitted to histologic and histomorphometric analyses.

Results: Data were analyzed statistically by two-way ANOVA and by the Tukey test. The interaction effect (ANOVA, F df(6; 48) = 4.64; p = 0.001 < 0.05) indicated that the relationship between treatments was not the same at each time of sacrifice. Although statistical analysis of the histomorphometric data showed a similar results for the treated and control groups. But histological analysis showed complete filling of the surgical defect throughout its extent was only for the group treated with Plumbum met.

Conclusion: The study demonstrated that for repair of surgical defects in rat mandibles Plumbum met. 30c and control did not differ significantly in histomorphometric terms.

Keywords

Calcitonin - bone regeneration - homeopathy - Plumbum metallicum
 

  • References

  • 1 Sweeney T.M., Opperman L.A., Persing J.A., Ogle R.C. Repair of critical size rat calvarial defects using extracellular matrix protein gels. J Neurosurg 1995; 83: 710-715.
    CrossrefPubMedGoogle Scholar
  • 2 Husseman F., Wolff O. A imagem do homem como base da arte médica: esboço de uma medicina orientada pela Ciência Espiritual. São Paulo: Associação Beneficiente Tobias; 1992. pp. 408–414
    Google Scholar
  • 3 Ganong W.F. Fisiologia médica. São Paulo: Atheneu; 1989. pp. 333–444
    Google Scholar
  • 4 Begum R., Koshy R., Sengupta A. Effect of homeopathic drugs plumbum and opium on experimentally induced lead toxicity in rats. Indian J Exp Biol 1994; 32: 192-195.
    PubMedGoogle Scholar
  • 5 Fisher P., House I., Belon P., Turner P. The influence of the homeopathic remedy plumbum metallicum on the excretion kinetics of lead in rats. Hum Toxicol 1987; 6: 321-324.
    CrossrefPubMedGoogle Scholar
  • 6 Arisawa E.A.L., Rocha R.F., Carvalho Y.R., Moraes E., Almeida J.D. Influência da calcitonin na reparação óssea de tíbias de ratas ovariectomizadas. Pós-Grad Rev Fac Odontol São José dos Campos 2000; 3: 54-59.
    PubMedGoogle Scholar
  • 7 Arisawa E.A.L., Brandão A.A.H., Almeida J.D., Rocha R.F. Calcitonin in bone-guided regeneration of mandibles in ovariectomized rats: densitometric, histologic and histomorphometric analysis. Int J Oral Maxillofac Surg 2008; 37: 47-53.
    CrossrefPubMedGoogle Scholar
  • 8 Pereira S.L.S., Toledo S., Okamoto T., Sallum E.A., Nociti Jr. F.H. Efeito da calcitonina de salmão sobre a cicatrização de defeitos ósseos. Rev Odontol UNESP (São Paulo) 1997; 26: 471-488.
    PubMedGoogle Scholar
  • 9 Reginster J.Y., Azria M., Gaspar S. et al. Endogenous production of specific antibodies does not decrease hypocalcemic response to calcitonin in young rabbits. Calcif Tissue Int 1992; 50: 518-520.
    CrossrefPubMedGoogle Scholar
  • 10 Ziegler R., Delling G. Effect of calcitonin on the regeneration of a circumscribed bone defect (bored hole in the rat tibia). Acta Endocrinol 1972; 69: 497-506.
    PubMedGoogle Scholar
  • 11 Weiss R.E., Singer F.R., Gorn A.H., Hofer D.P., Nimni M.E. Calcitonin stimulates bone formation when administered prior to initiation of osteogenesis. J Clin Invest 1981; 68: 815-818.
    CrossrefPubMedGoogle Scholar
  • 12 Aires M.M. Fisiologia. 2nd edn. Rio de Janeiro: Guanabara Koogan; 1999. pp. 855–876
    Google Scholar
  • 13 Gomes M.F., Anjos M.J.S., Nogueira T.O., Tde O., Catanzaro Guimarães S.A. Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies. Int J Oral Maxillofac Implants 2002; 17: 488-497.
    PubMedGoogle Scholar
  • 14 Linde A., Thoren C., Dahlin C., Sandberg E. Creation of new bone by an osteopromotive membrane technique: an experimental study in rats. J Oral Maxillofac Surg 1993; 51: 892-897.
    CrossrefPubMedGoogle Scholar
  • 15 Linde A., Alberius P., Dahlin C., Bjurstam K., Sundin Y. Osteopromotion: a soft-tissue exclusion principle using a membrane for bone healing and bone neogenesis. J Periodontol 1993; 64: 1116-1128.
    CrossrefPubMedGoogle Scholar
  • 16 Pecora G., Andreana S., Margarone 3rd J.E., Covanl U., Sottosanti J. Bone regeneration with a calcium sulfate barrier. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997; 84: 424-429.
    CrossrefPubMedGoogle Scholar
  • 17 Szachowicz E.H. Facial bone wound healing. Otolaryngol Clin North Am 1995; 28: 865-881.
    PubMedGoogle Scholar
  • 18 Almeida J.D., Carvalho Y.R., Rocha R.F., Arisawa E.A.L. Estudo da reparação óssea em mandíbula de ratos. Pós-Grad Rev Fac Odontol São José dos Campos 2000; 3: 49-53.
    PubMedGoogle Scholar
  • 19 Almeida J.D., Arisawa E.A.L., Lima A.P. et al. Effect of calcitonin on bone regeneration in male rats: a histomorphometric analysis. Int J Oral Maxillofac Surg 2007; 36: 435-440.
    CrossrefPubMedGoogle Scholar
  • 20 Almeida J.D., Arisawa E.A.L., Lima A.P., Rocha R.F., Carvalho Y.R. Estudo do efeito do Plumbum mettalicum 30CH na reparação óssea em mandíbula de ratos sem barreira mecânica. Cienc Odontol Bras 2006; 9: 69-76.
    PubMedGoogle Scholar
  • 21 Dahlin C., Linde A., Gottlow J., Nyman S. Healing of bone defects by guided tissue regeneration. Plast Reconstr Surg 1988; 81: 672-676.
    CrossrefPubMedGoogle Scholar
  • 22 Dahlin C., Sandberg E., Alberius P., Linde A. Restoration of mandibular nonunion bone defects. Int J Oral Maxillofac Surg 1994; 23: 237-242.
    CrossrefPubMedGoogle Scholar
  • 23 Sandberg E., Dahlin C., Linde A. Bone regeneration by the osteopromotion technique using bioabsorbable membranes: an experimental study in rats. J Oral Maxillofac Surg 1993; 51: 1106-1114.
    CrossrefPubMedGoogle Scholar
  • 24 Raubenheimer EJ. Recent advances in the diagnosis and management of metabolic bone diseases. In: International Congress of the International Association of Oral Pathology, 2000, pp. 1–14.
    PubMed
  • 25 Badurski J.E., Schwamm W., Popko J., Zimnoch L., Rogowski F., Pawlica J. Chondroprotective action of salmon calcitonin treatment in experimental arthropathies. Calcif Tissue Int 1991; 49: 27-34.
    CrossrefPubMedGoogle Scholar
  • 26 Farley J.R., Tarbaux N.M., Linkhart T.A., Baylink D.J. The anti-bone-resorptive agent calcitonin also acts in vitro to directly increase bone formation and bone cell proliferation. Endocrinology 1988; 123: 159-167.
    CrossrefPubMedGoogle Scholar