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Thromb Haemost 2017; 117(01): 116-126
DOI: 10.1160/TH16-07-0564
DOI: 10.1160/TH16-07-0564
Cellular Haemostasis and Platelets
The Hippo pathway regulates human megakaryocytic differentiation
Financial support: This research project was funded by grants from Thailand Research Fund (grant no. RTA 488–0007) and the Commission on Higher Education (grant no. CHE-RES-RG-49). S. Issaragrisil is a senior research scholar of Thailand Research Fund.
Further Information
Publication History
Received:
22 July 2017
Accepted after major revision:
29 September 2017
Publication Date:
01 December 2017 (online)
Summary
The Hippo pathway is involved in several biological processes in both flies and mammals. Recent studies have shown that the Hippo pathway regulates Drosophila’s haematopoiesis; however, understanding of its role in mammalian haematopoiesis is still limited. In flies, deletion of the Hippo component gene, Warts, affects crystal cell differentiation. We explored the role of the Hippo pathway in human haemato-poiesis focusing on megakaryopoiesis. To investigate the role of LATS1/2 (a mammalian homolog of Warts) in human megakaryo -blastic cell differentiation and platelet formation, megakaryoblastic cell (MEG-01) line was used as a model to gain insight into mechan-ism of the Hippo pathway in mammalian megakaryopoiesis. Effect of LATS1/2 on megakaryoblastic cell differentiation and platelet production were determined by functional changes. We found that depletion of LATS1/2 resulted in an increase of CD41+ megakaryocytes with impaired platelet biogenesis. Our study shows that the Hippo signalling pathway plays a crucial role in human megakaryoblastic cell differentiation and thrombopoiesis.
Supplementary Material to this article is available online at www.thrombosis-online.com.
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