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Thromb Haemost 2007; 97(02): 218-227
DOI: 10.1160/TH06-05-0257
Platelets and Blood Cells
Schattauer GmbH

Platelet activation by collagen is increased in retinal vein occlusion

Giuliana Leoncini
1   Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Genova, Genova, Italy
,
Debora Bruzzese
1   Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Genova, Genova, Italy
,
Maria Grazia Signorello
1   Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Genova, Genova, Italy
,
Ugo Armani
2   Centro di Ricerca sulla Trombosi et Aterosclerosi, Dipartimento di Medicina Interna, Università di Genova, Genova, Italy;
,
Antonietta Piana
2   Centro di Ricerca sulla Trombosi et Aterosclerosi, Dipartimento di Medicina Interna, Università di Genova, Genova, Italy;
,
Davidina Ghiglione
3   Dipartimento di Neuroscienze, Oftalmologia e Genetica, Università di Genova, Genova, Italy
,
Paola Camicione
3   Dipartimento di Neuroscienze, Oftalmologia e Genetica, Università di Genova, Genova, Italy
› Author AffiliationsFinancial support: This work was supported by a grant from the Ministero della Salute, Rome, Italy.
Further Information

Publication History

Received 11 May 2006

Accepted after resubmission 11 January 2006

Publication Date:
25 November 2017 (online)

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Summary

Retinal vein occlusion (RVO) is the most common retinal vascular disorder second to diabetic retinopathy. The main risk factors in patients with RVO are hypertension, diabetes, hyperlipidemia, increased blood viscosity and glaucoma. The pathogenesis of RVO has not yet been clarified. In these events platelets could play a very important role. In the present study the platelet response to collagen was deeply investigated. Experiments were carried out on a selected group of RVO patients, which were compared to a group of healthy subjects matched for age, sex, clinical and metabolic characteristics. In resting and activated platelets of both groups of subjects p72syk phosphorylation, phospholipase Cγ 2 phosphorylation, protein kinase C activation, intracellular calcium levels and nitric oxide formation were measured. Results show that platelets of patients were more responsive to collagen or ADP than healthy subjects and that the response was significantly different (p < 0.0005) at low concentrations of these agonists. In platelets of patients stimulated with collagen increased phosphorylation of p72syk and phospholipase C γ 2 was found. Also protein kinase C was more activated in patients. In addition intracellular calcium rise induced by collagen was significantly higher in patients than in healthy subjects. RVO patients showed a lower basal level of nitric oxide both in resting and stimulated platelets compared to healthy subjects. Altogether these results suggest that the platelet hyperaggregability described in patients might be an important factor in the development of RVO contributing to the thrombogenic effects.

Keywords

Collagen - human platelets - retinal vein occlusion
 

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