Thromb Haemost 2005; 94(06): 1230-1235
DOI: 10.1160/TH05-05-0344
Platelets and Blood Cells
Schattauer GmbH

Neutrophil P-selectin-glycoprotein-ligand-1 binding to platelet P-selectin enhances metalloproteinase 2 secretion and platelet-neutrophil aggregation

Haissam Abou-Saleh
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
,
Jean-François Théorêt
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
,
Daniel Yacoub
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
,
Yahye Merhi
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
› Author Affiliations
Financial support: Supported by the Canadian Institutes of Health Research (CIHR) and the Heart and Stroke Foundation of Canada (HSFC).
Further Information

Publication History

Received 18 May 2005

Accepted after resubmission 07 October 2005

Publication Date:
07 December 2017 (online)

Summary

Platelets and neutrophils constitute a high source of metalloproteinases (MMPs), and their interactions via P-selectin and Pselectin- glycoprotein-ligand-1 (PSGL-1) are involved in thrombosis, vascular remodelling, and restenosis. We investigated the impact of these interactions on platelet MMP-2 secretion and function in platelet and neutrophil aggregation. The secretion of MMP-2 from human platelets was significantly increased threefold after thrombin activation, and enhanced two-fold in the presence of neutrophils. Neutrophil supernatant had no effect on platelet MMP-2 secretion. While no MMP-2 was detected in the supernatant of neutrophils, a high amount of MMP-9 was released by neutrophils, and remained unchanged upon thrombin activation or in the presence of platelets. Platelet P-selectin, which increased significantly after activation, triggered platelet binding to neutrophils that was completely inhibited by P-selectin or PSGL-1 antagonists, and was reduced by 50% with a GPIIb/ IIIa antagonist. P-selectin or PSGL-1 antagonism abolished the enhanced secretion of platelet MMP-2 in the presence of neutrophils and reduced platelet-neutrophil aggregation. Platelet activation and binding to neutrophils enhance the secretion of platelet MMP-2 via an adhesive interaction between P-selectin and PSGL-1, which contribute to increase platelet-neutrophil aggregation.

 
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