Biosynthesis of Polyacetylenes in Ambrosia maritima Hairy Roots

Abstract

Thiophene A and thiophene A diol are the major polyacetylenes isolated from the hairy roots of Ambrosia maritima (Asteraceae) cultured under continuous light irradiation. The biosynthesis of thiophene A was studied using [1 - ¹³C]-, [2 - ¹³C]-, [1,2 - ¹³C]-acetates. The biosynthesis of thiarubrine A, produced by hairy roots cultured in the dark, was studied using [1,2 - ¹³C]-acetates and [18 - ¹³C]-linoleic acid. Our results suggest a catabolic pathway for polyacetylenes biosynthesis from linoleic acid in hairy roots of Ambrosia maritima.

Abbreviations

DEPT:distortionless enhancement of polarization transfer

HMBC:heteronuclear multiple correlation

MeJ:methyl jasmonate

MS:Murashige and Skoog medium

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