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Planta Med 2006; 72(12): 1149-1156
DOI: 10.1055/s-2006-947194
Original Paper
Physiology and in vitro Biotechnology
© Georg Thieme Verlag KG Stuttgart · New York

Cardenolide Genin Pattern in Isoplexis Plants and Shoot Cultures

Frank Schaller1 , Wolfgang Kreis1
  • 1Lehrstuhl für Pharmazeutische Biologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany
In memory of Professor Ernst Reinhard
Further Information

Publication History

Received: March 28, 2006

Accepted: June 8, 2006

Publication Date:
10 August 2006 (online)

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Abstract

An HPLC method for the quantitative analysis of cardenolides was developed and applied. The procedure was optimised for analysing small samples (40 mg dw) of plant and tissue culture material. Isoplexis canariensis plants obtained from seeds accumulated cardenolides to about 20 - 40 μmol g-1 dw as calculated from the levels of cardenolide genins released after acidic hydrolysis of methanolic extracts. The relative contents of xysmalogenin, digitoxigenin, uzarigenin and canarigenin were 5 - 15 %, 0 - 5 %, 10 - 15 % and 70 - 90 %, respectively. Shoot cultures were initiated from seeds, established as permanent cultures and cultivated on agar-solidified or in liquid medium. Shoot cultures maintained on solid medium contained an average of about 6 μmol cardenolides g-1 dw. A relatively high proportion of digitoxigenin was found in two-thirds of the shoot cultures examined. The cardenolide content of amphibian shoot cultures averaged to about 1 μmol g-1 dw. Plants regenerated from shoot cultures and maintained under hydroponic conditions accumulated the same amount of cardenolides as plants collected in the field. No cardenolides could be detected in callus cultures.

Key words

Isoplexis canariensis - Plantaginaceae - cardenolides - cardiac glycosides - uzarigenin - xysmalogenin - digitoxigenin - canarigenin - dianhydroperiplogenin - shoot culture - callus culture - hydroponics

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Prof. Dr. Wolfgang Kreis

Institut für Biologie

Lehrstuhl Pharmazeutische Biologie

Friedrich-Alexander-Universität Erlangen-Nürnberg

Staudtstr. 5

91058 Erlangen

Germany

Phone: +49-9131-852-8241

Fax: +49-9131-852-8243

Email: wkreis@biologie.uni-erlangen.de