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Planta Med 2006; 72(8): 708-714
DOI: 10.1055/s-2006-931602
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Chrysin Inhibits Lipopolysaccharide-Induced Angiogenesis via Down-Regulation of VEGF/VEGFR-2(KDR) and IL-6/IL-6R Pathways

Chiu-Mei Lin1 , 2 , Hang Chang3 , 4 , Shih-Yun Li1 , I-Hsing Wu5 , Jen-Hwey Chiu1 , 6 , 7
  • 1Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
  • 2Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
  • 3Department of Emergency Medicine and Cardiovascular Surgery, Taipei City Hospital, Taiwan, R.O.C.
  • 4College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C.
  • 5Central Laboratory, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
  • 6Division of General Surgery, Department of Surgery, Veterans General Hospital, Taipei, Taiwan, R.O.C.
  • 7Cheng-Hsing Rehabilitation Medicine Center, Taipei, Taiwan, R.O.C.
Further Information

Publication History

Received: December 21, 2005

Accepted: March 23, 2006

Publication Date:
29 May 2006 (online)

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Abstract

The relationship between chrysin and inflammation-induced angiogenesis remains unclear. The aim of this study was to evaluate the suppressive effects of chrysin on lipopolysaccharide (LPS)-induced angiogenesis in chicken chorioallantoic membrane (CAM) as well as in human umbilical endothelial cells (HUVEC). The in vivo CAM model was applied to evaluate the percentage of new vessels formation, followed by measuring endothelial migration and tube formation in HUVEC cultures. The mechanisms of the suppressive effect of chrysin on LPS-induced angiogenesis, in terms of VEGF, VEGF receptors (VEGFR), interleukin 6 (IL-6) and IL-6 receptor gene expressions, were analyzed by Western blot, ELISA cytokine assay, and quantitative real time PCR. The results showed that chrysin (10 - 8 - 10 - 5M) inhibited LPS-induced CAM neovascular density. There was a significant down-regulation of VEGF and VEGFR-2 (KDR) but not VEGFR-1 (Flt-1) gene expression by chrysin in LPS-treated HUVEC cultures. Besides, chrysin concentration-dependently inhibited the auto-regulation loop of IL-6/IL-6R in LPS-treated HUVEC cells. We conclude that chrysin suppresses both in vitro and in vivo LPS-induced angiogenesis.

Key words

Lipopolysaccharide - angiogenesis - chrysin - VEGF - KDR

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Jen-Hwey Chiu, MD, PhD

Institute of Traditional Medicine

School of Medicine

National Yang-Ming University

155, Sec. 2, Li-Nong St.

Peitou

Taipei 112

Taiwan

Republic of China

Phone: +886-2-2826+7178

Fax: 886-2-2822-5044

Email: chiujh@mailsrv.ym.edu.tw

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