Abstract
Echinacea purpurea cell cultures have been established for the production of secondary and possibly
bioactive compounds using elicitation techniques. Different elicitors including yeast
elicitor, methyl jasmonate, glutathione, and manganese ions were used and compared
for their effects on the formation of phenolics in the cell cultures. The accumulation
of phenolics in the medium after elicitation with glutathione and manganese ions showed
similar patterns as detected by HPLC in control medium. However, in yeast elicitor-
and methyl jasmonate-treated cell suspension cultures, other phenolics were formed
and accumulated in the medium. In contrast, both control and elicited cells contained
the same pattern of phenolics. The main phenolics both in the medium and cells, 19
in total, were isolated and identified on the basis of chromatographic, chemical (derivatisation),
enzymatic and spectroscopic techniques. The medium contained lignans, neolignans and
acetophenone derivatives as main elicitor-enhanced products. The cells mainly contained
phenolic glycosides including a new compound, α-O-β-D-glucopyranosylacetovanillone. Detailed collision-induced dissociation electrospray
ionisation mass spectrometric fragmentation pathways for 8,4′-oxyneolignan glycosides
are discussed.
Key words
Echinacea purpurea
- cell suspension cultures - elicitation - neolignans - acetophenones
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Dr. Wen-Wu Li
Institut für Biologie II/Biochemie
Albert-Ludwigs-Universität Freiburg
Schänzlestrasse 1
79104 Freiburg
Germany
Fax: +49-761-203-2601
Email: wenwu.li@biologie.uni-freiburg.de