Planta Med 2006; 72(2): 121-125
DOI: 10.1055/s-2005-373168
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Antiviral Activity of a Carrageenan from Gigartina skottsbergii against Intraperitoneal Murine Herpes simplex Virus Infection

C. A. Pujol1 , L. A. Scolaro1 , M. Ciancia2 , M. C. Matulewicz2 , A. S. Cerezo2 , E. B. Damonte1
  • 1Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
  • 2Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Further Information

Publication History

Received: January 12, 2005

Accepted: June 27, 2005

Publication Date:
10 November 2005 (online)

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Abstract

The partially cyclized μ/ν-carrageenan 1C3, isolated from the red seaweed Gigartina skottsbergii, was previously shown to be a potent inhibitor of the in vitro replication of Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Here the protective effect of 1C3 in a murine model of intraperitoneal (i. p.) HSV-1 infection was evaluated. OF1 mice were i. p. infected with 5 × 105 PFU of HSV-1 KOS strain, and the effects of different treatments with 1C3 were studied. When 30 mg/kg of body weight of 1C3 was administered by the i. p. route immediately after HSV-1 infection, 87.5 % survival of the animals was achieved (p < 0.005), associated with a delay in the mean day of death in 1C3-treated non-surviving mice. Animal survival was not improved when multiple doses of 1C3 were also given in the period 1 - 48 h post-infection, and no protection was afforded when treatment was started after 24 h of infection. When virus and compound were injected by different routes, i. p. and intravenous (i. v.), respectively, a still significant protection was achieved (40 % survival, p < 0.05). No toxicity of 1C3 for the animals was recorded. The pharmacokinetic properties were analyzed after injection of 1C3 into the tail vein by monitoring of [3 H]-1C3 in plasma and organs and by a bioassay of the anti-HSV-1 activity remaining in serum after non-radioactive 1C3 inoculation. A very rapid disappearance of the compound from the blood was observed since only 5.9 - 0.9 % of the radioactivity of the initially administered [3 H]-1C3 appeared in the plasma between 5 - 300 minutes after administration. A transient peak of radioactivity was detected in the kidney 15 minutes after inoculation. The bioassay confirms the presence of the compound circulating in a biologically active form up to 1 hour after injection.

References

Carlos Alberto Pujol

Laboratorio de Virología

Departamento de Química Biológica

Facultad de Ciencias Exactas y Naturales

Universidad de Buenos Aires

Pabellón 2

Piso 4

Ciudad Universitaria

C1428BGA Buenos Aires

Argentina

Phone: +54-11-4576-3334

Fax: +54-11-4576-3342

Email: capujol@qb.fcen.uba.ar