Platelet Anti-Aggregating Triterpenoids from the Leaves of Acanthopanax senticosus and the Fruits of A. sessiliflorus

Abstract

Six triterpenoids, chiisanogenin, chiisanoside, ursolic acid, oleanolic acid, β-sitosterol and daucosterol, were isolated as the platelet anti-aggregating components from the leaves of Acanthopanax senticosus and the fruits of A. sessiliflorus. Chiisanogenin showed about 50-fold higher potency than acetylsalicylic acid (ASA) on U46619-induced platelet aggregation (IC₅₀: 6.21 μM) and 10 - 20-fold higher effects than ASA on epinephrine- and arachidonic acid (AA)-induced aggregation (IC₅₀: 2.50 and 4.81 μM, respectively).

References

• 1 Yun-Choi H S, Kim J H, Lee J R. Potential inhibitors of platelet aggregation from plant sources. III.  J Nat Prod. 1987;  50 1059-64
• 2 Yun-Choi H S, Kim S O, Kim J H, Lee J R, Cho H I. Modified smear method for screening potential inhibitors of platelet aggregation from plant sources.  J Nat Prod. 1985;  48 363-70
• 3 Yun-Choi H S, Park K M, Pyo M K. Epinephrine induced platelet aggregation in rat platelet-rich plasma.  Thromb Res. 2000;  100 511-8
• 4 Pyo M K, Yun-Choi H S, Hong Y -J. Antiplatelet activities of aporphine alkaloids isolated from leaves of Magnolia obovata .  Planta Med. 2003;  69 267-9
• 5 Rand M L, Leung R, Packham M A. Platelet function assays.  Transfusion Apheresis Sci. 2003;  28 307-17
• 6 Hahn D R, Kasai R, Kim J H, Taniyasu S, Tanaka O. A new glycosyl ester of a 3,4-seco-triterpene from Korean medicinal plant, Acanthopanax chiisanensis (Araliaceae).  Chem Pharm Bull. 1984;  32 1244-7
• 7 Shirasuna K, Miyakoshi M, Mimoto S, Isoda S, Satoh Y. et al . Lupane triterpenoid glycosyl esters from leaves of Acanthopanax divaricatus .  Phytochemistry. 1997;  45 579-84
• 8 Lee S, Shin D -S, Oh K -B, Shin K H. Antibacterial compounds from the leaves of Acanthopanax senticosus .  Arch Pharm Res. 2003;  26 40-2
• 9 Lee S, Kim B K, Cho S H, Shin K H. Phytochemical constituents from the fruits of Acanthopanax sessiliflorus .  Arch Pharm Res. 2002;  25 280-4
• 10 Mahato S B, Kundu A P. ¹³C NMR spectra of pentacyclic triterpenoids - A compilation and some salient features.  Phytochemistry. 1994;  37 1517-75
• 11 Chang I -M, Yun (Choi)  HS, Yamasaki K. Revision of ¹³C NMR assignments of β-sitosterol and β-sitosteryl-3-O-β-D-glucopyranoside isolated from Plantago asiatica seed.  Kor J Pharmacog. 1981;  12 12-4
• 12 Rubinstein I, Goad L J, Clague A DH, Mulheirn L J. The 220 MHz NMR spectra of phytosterols.  Phytochemistry. 1976;  15 195-200
• 13 McNicol A. Platelet preparation and estimation of functional responses. In: Watson SP, Authi KS, editors Platelet-A Practical approach. Oirl Press at Oxford University Press 1996: pp 1-26