Leukocyte Heat Shock Protein Expression Before and After Intensified Training

Abstract

The purpose of the current research was to test the hypothesis that exercise induced leukocyte heat shock protein (HSP) expression is increased during periods of intensified exercise training. Seven male endurance cyclists carried out tests of maximal oxygen consumption and endurance capacity. These standard exercise tests were carried out prior to and following 6 days of prescribed intensified training. Sampled leukocytes were examined for Hsp27 and Hsp70 expression using a Fluorescence Activated Cell Scanner (EPICS XL, Coulter). During a period of overreaching, as signified by a drop in time to fatigue following the intensified training period (p = 0.02), the number of extracellular Hsp27 positive granulocytes increased in response to the VO₂max test. Acute, intracellular HSP responses were observed in both baseline and overreached conditions.The present study showed that a period of intensified training caused adaptations in the acute heat shock protein exercise response, reflected by a greater increase of cell surface HSP positive leukocytes following heavy training.

Key words

Cycling - overreaching - cell protection

References

• 1 Arnold-Schild D, Hanau D, Spehner D, Schmid C, Rammensee H G, de la Salle S H, Schild H. Cutting edge: receptor-mediated endocytosis of heat shock proteins by professional antigen-presenting cells.  J Immunol. 1999;  162 3757-3760
• 2 Asea A, Kraeft S K, Kurt-Jones E A, Stevenson M A, Chen L B, Finberg R W, Koo G C, Calderwood S K. HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine.  Nat Med. 2000;  6 435-442
• 3 Breloer M, Dorner B, More S H, Roderian T, Fleischer B, von Bonin A. Heat shock proteins as “danger signals”: eukaryotic Hsp60 enhances and accelerates antigen-specific IFN-gamma production in T cells.  Eur J Immunol. 2001;  31 2051-2059
• 4 Chen W, Syldath U, Bellmann K, Burkart V, Kolb H. Human 60-kDa heat-shock protein: a danger signal to the innate immune system.  J Immunol. 1999;  162 3212-3219
• 5 Febbraio M A, Koukoulas I. HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise.  J Appl Physiol. 2000;  89 1055-1060
• 6 Febbraio M A, Ott P, Nielsen H B, Steensberg A, Keller C, Krustrup P, Secher N H, Pedersen B K. Exercise induces hepatosplanchnic release of heat shock protein 72 in humans.  J Physiol. 2002;  544 957-962
• 7 Febbraio M A, Steensberg A, Walsh R, Koukoulas I, van Hall G, Saltin B, Pedersen B K. Reduced glycogen availability is associated with an elevation in HSP72 in contracting human skeletal muscle.  J Physiol. 2002;  538 911-917
• 8 Fehrenbach E, Niess A M. Role of heat shock proteins in the exercise response.  Exerc Immunol Rev. 1999;  5 57-77
• 9 Fehrenbach E, Niess A M, Schlotz E, Passek F, Dickhuth H H, Northoff H. Transcriptional and translational regulation of heat shock proteins in leukocytes of endurance runners.  J Appl Physiol. 2000;  89 704-710
• 10 Fehrenbach E, Passek F, Niess A M, Pohla H, Weinstock C, Dickhuth H H, Northoff H. HSP expression in human leukocytes is modulated by endurance exercise.  Med Sci Sports Exerc. 2000;  32 592-600
• 11 Lanier L L, Warner N L. Paraformaldehyde fixation of hematopoietic cells for quantitative flow cytometry (FACS) analysis.  J Immunol Methods. 1981;  47 25-30
• 12 Lehmann M J, Lormes W, Opitz-Gress A, Steinacker J M, Netzer N, Foster C, Gastmann U. Training and overtraining: an overview and experimental results in endurance sports.  J Sport Med Phys Fitn. 1997;  37 7-17
• 13 Liu Y, Lormes W, Baur C, Opitz-Gress A, Altenburg D, Lehmann M, Steinacker J M. Human skeletal muscle HSP70 response to physical training depends on exercise intensity.  Int J Sport Med. 2000;  21 351-355
• 14 Liu Y, Steinacker J M. Changes in skeletal muscle heat shock proteins: pathological significance.  Front Biosci. 2001;  6 12-25
• 15 Locke M. The cellular stress response to exercise: role of stress proteins.  Exerc Sport Sci Rev. 1997;  25 105-136
• 16 Locke M, Noble E G, Atkinson B G. Exercising mammals synthesize stress proteins.  Am J Physiol. 1990;  258 723-729
• 17 Matzinger P. An innate sense of danger.  Semin Immunol. 1998;  10 399-415
• 18 Niess A M, Passek F, Lorenz I, Schneider E M, Dickhuth H H, Northoff H, Fehrenbach E. Expression of the antioxidant stress protein heme oxygenase-1 (HO-1) in human leukocytes.  Free Rad Bio Med. 1999;  26 184-192
• 19 Ryan A J, Gisolfi C V, Moseley P L. Synthesis of 70 K stress protein by human leukocytes: effect of exercise in the heat.  J Appl Physiol. 1991;  70 466-471
• 20 Shastry S, Toft D O, Joyner M J. HSP70 and HSP90 expression in leucocytes after exercise in moderately trained humans.  Acta Physiol Scand. 2002;  175 139-146
• 21 Skidmore R, Gutierrez J A, Guerriero Jr V, Kregel K C. HSP70 induction during exercise and heat stress in rats: role of internal temperature.  Am J Physiol. 1995;  268 92-97
• 22 Snoeckx L H, Cornelussen R N, Van Nieuwenhoven F A, Reneman R S, Van Der Vusse G J. Heat shock proteins and cardiovascular pathophysiology.  Physiol Rev. 2001;  81 1461-1497
• 23 Srivastava P. Interaction of heat shock proteins with peptides and antigen presenting cells: chaperoning of the innate and adaptive immune response.  Annu Rev Immunol. 2002;  20 395-425
• 24 Urhausen A, Kindermann W. Diagnosis of overtraining: what tools do we have?.  Sport Med. 2002;  32 95-102
• 25 Walsh R C, Koukoulas I, Garnham A, Moseley P L, Hargreaves M, Febbraio M A. Exercise increases serum Hsp72 in humans.  Cell Stress Chaperones. 2001;  6 386-393