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Int J Sports Med 2003; 24(7): 530-534
DOI: 10.1055/s-2003-42010
Nutrition
© Georg Thieme Verlag Stuttgart · New York

Protective Effect of Sesamin Administration on Exercise-Induced Lipid Peroxidation

T.  Ikeda1 , Y.  Nishijima2 , H.  Shibata2 , Y.  Kiso2 , K.  Ohnuki3 , T.  Fushiki1 , T.  Moritani1
  • 1Laboratory of Applied Physiology, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan
  • 2Institute for Health Care Science, Suntory Ltd., Osaka, Japan
  • 3Laboratory of Nutrition Chemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Further Information

Publication History

Accepted after revision: January 30, 2003

Publication Date:
10 September 2003 (online)

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Abstract

To investigate the mechanism of antioxidative effects of sesamin in vivo, 32 male ddY mice were administered with 10 mg/kg or 100 mg/kg of sesamin (S10, S100), 100 mg/kg of vitamin E (VE100) or control sample (C). They were subjected to 30 min of swimming exercise 2 h after the sample administration by using a new forced-swimming apparatus, i. e. an adjustable-current swimming pool. Exercise resulted in a significant increase in plasma lipid peroxides (LPO) in C and VE100 (p < 0.01), but not in S10 and S100. S100 showed significantly higher total glutathione peroxidase activity and glutathione S-transferase activity in liver compared to C (p < 0.05). In conclusion, sesamin may enhance LPO degradation in the liver resulting in the strong protective effects against exercise-induced plasma lipid peroxidation.

Key words

Glutathione S-transferase - lipid peroxides - sesamin - vitamin E - mice - exercise

References

  • 1 Ahn D, Putt D, Kresty L, Stoner G D, Fromm D, Hollengerg P F. The effects of dietary ellagic acid on rat hepatic and esophageal mucosal cytochromes P450 and phase II enzymes.  Carcinogenesis. 1996;  17 821-828
    PubMedGoogle Scholar
  • 2 Asami S, Akimoto K, Abe K, Akamatsu T, Konishi K, Shimizu S, Sugano M, Yamada H. Antioxidant activity of sesamin on NADPH-dependent lipid peroxidation in liver microsomes.  Nippon Nogeikagaku Kaishi. 1993;  67 265
    PubMedGoogle Scholar
  • 3 Barakat H A, Dohm G L, Shukla N. Influence of age and exercise training on lipid metabolism in Fischer-344 rats.  J Appl Physiol. 1989;  67 1638-1642
    PubMedGoogle Scholar
  • 4 Barnett C R, Abbott R A, Bailey C J, Flatt P R, Ioannides C. Cytochrome P450-dependent mixed-function oxidase and glutathione S-transferase activities in spontaneous obesity-diabetes.  Biochem Pharmacol. 1992;  43 1868-1871
    CrossrefPubMedGoogle Scholar
  • 5 Carrilo M, Nokubo M, Kitani K, Satoh K, Sato K. Age-related alterations of enzyme activities and subunits of hepatic glutathione S-transferases in male and female Fischer-344 rats.  Biochimica et Biophysica Acta. 1991;  1077 325-331
    PubMedGoogle Scholar
  • 6 Davies K JA, Quintanilha A T, Brooks G A, Packer L. Free radicals and tissue damage produced by exercise.  Biochem Biophys Res Commun. 1982;  107 1198-1205
    CrossrefPubMedGoogle Scholar
  • 7 Dixon Z R, Shie F, Warden B A, Burri B J, Neidlinger T R. The effect of a low carotenoid diet on malondialdehyde-thiobarbituric acid (MDA-TBA) concentrations in women: a placebo-controlled double-blind study.  J Am Coll Nutri. 1998;  17 54-58
    PubMedGoogle Scholar
  • 8 Faure M, Lissi E, Torres R, Videla L A. Antioxidant activities of lignans and flavonoids.  Phytochemistry. 1990;  29 3773-3775
    CrossrefPubMedGoogle Scholar
  • 9 Floyd R A. Role of oxygen free radicals in carcinogenesis and brain ischemia.  FASEB J. 1990;  4 2587-2597
    PubMedGoogle Scholar
  • 10 Fujiwara Y, Nishihara H, Nakayoshi T, Hasegawa T, Tagami S, Kawakami Y. Elevation of plasma lipid peroxides in non-insulin dependent diabetics with multiple lacunar infarcts.  J Atheroscler Thromb. 1997;  4 90-95
    PubMedGoogle Scholar
  • 11 Fukuda Y, Nagata M, Osawa T, Namiki M. Contribution of lignan analogues to antioxidative activity of refined unroasted sesame seed oil.  J Am Oil Chem Soc. 1986;  63 1027-1031
    PubMedGoogle Scholar
  • 12 Glavind J, Hartmann S, Clemmensens J, Jensen K E, Dam H. Studies on the role of lipoperoxides in human pathology II. The presence of peroxidized lipids in the arteriosclerotic aorta.  Acta Pathol Microb Scand. 1952;  30 1-6
    PubMedGoogle Scholar
  • 13 Habig W H, Jakoby W B. Assay for differentiation of glutathione S-transferase.  Meth Enzymol,. 1981;  77 398-405
    PubMedGoogle Scholar
  • 14 Habig W H, Pabst M J, Jakoby W B. Glutathione S-transferase; The first enzymatic step in mercapturic acid formation.  J Biol Chem. 1974;  249 7130-7139
    PubMedGoogle Scholar
  • 15 Harman D. Free radical theory of aging: role of free radicals in the origination and evolution of life, aging and disease processes. In: Johnson Jr. JE, Walford R, Harman D, Miquel J (eds) Free radicals, aging and degenerative Diseases. New York: Alan R. Liss; 1. Vol. 1986: 3-49
    Google Scholar
  • 16 Hirose N, Doi F, Ueki T, Akazawa K, Chijiiwa K, Sugano M, Akimoto K, Shimizu S, Yamada H. Suppressive effect of sesamin against 7,12-dimethylbenz[a]anthracene induced rat mammary carcinogenesis.  Anticancer Res. 1992;  12 1259-1266
    PubMedGoogle Scholar
  • 17 Kanter M M, Nolte L A, Holloszy J O. Effects of an antioxidant vitamin mixture on lipid peroxidation at rest and postexercise.  J Appl Physiol. 1993;  74 965-969
    CrossrefPubMedGoogle Scholar
  • 18 Kim K, Kawada T, Ishihara K, Inoue K, Fushiki T. Swimming capacity of mice is increased by oral administration of a nonpungent capsaicin analog, stearoyl vanillylamide.  J Nutr. 1998;  128 1978-1983
    PubMedGoogle Scholar
  • 19 Lawrence R A, Burk R F. Species, tissue and subcellular distribution of non Se-dependent glutathione peroxidase activity.  J Nutri. 1978;  108 211-215
    PubMedGoogle Scholar
  • 20 Matsumoto K, Ishihara K, Tanaka K, Inoue K, Fushiki T. An adjustable-current swimming pool for the evaluation of endurance capacity of mice.  J Appl Physiol. 1996;  81 1843-1849
    PubMedGoogle Scholar
  • 21 Meydani M, Evans W J, Handelman G, Biddle L, Fielding R A, Meydani S N, Burrill J, Fiatarone M A, Blumberg J B, Cannon J G. Protective effect of vitamin E on exercise-induces oxidative damage in young and older adults.  Am J Physiol. 1993;  264 992-998
    PubMedGoogle Scholar
  • 22 Miquel J, Ramirez-Bosca A, Soler A, Diez A, Carrion-Gutierrez M A, Diaz-Alperi J, Quintanilla-Ripoll E, Bernd A, Quintanilla-Almagro E. Increase with age of serum lipid peroxides: implications for the prevention of atherosclerosis.  Mechanisms of aging and development. 1998;  100 17-24
    PubMedGoogle Scholar
  • 23 Ohishi N, Ohkawa H, Miike A, Tatano T, Yagi K. A new assay method for lipid peroxides using a methylene blue derivative.  Biochemistry international. 1985;  10 205-211
    PubMedGoogle Scholar
  • 24 Paglia D E, Valentine W N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.  J Lab Clin Med. 1967;  70 158-169
    PubMedGoogle Scholar
  • 25 Prestera T, Holtzclaw W D, Zhang Y, Talalay P. Chemical and molecular regulation of enzymes that detoxify carcinogens.  Proc Natl Acad Sci USA. 1993;  90 2965-2969
    CrossrefPubMedGoogle Scholar
  • 26 Redinbaugh M G, Turley R B. Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions.  Anal Biochem. 1986;  153 267-271
    CrossrefPubMedGoogle Scholar
  • 27 Santos M T, Valles J, Aznar J. Plasma lipid peroxides in patients with vascular disease and in middle-aged normal subjects with a high risk of atherosclerosis. In: Miquel J, Quintanilha A, Weber H (eds) CRC Handbook of Free Radicals and Antioxidants in Biomedicine. Boca Raton, FL: CRC Press; 1. Vol. 1989: 237-254
    Google Scholar
  • 28 Sen C K. Oxidants and antioxidants in exercise.  J Appl Physiol. 1995;  79 675-686
    PubMedGoogle Scholar
  • 29 Stringer M D, Gorog P G, Freeman A, Kakkar V V. Lipid Peroxides and atherosclerosis.  Br Med J. 1989;  298 281-283
    PubMedGoogle Scholar
  • 30 Sugita O, Okada M, Kimura S, Matsuto T. The improvement of colorimetric determination of lipid peroxide in human serum.  Jpn J Clin Pathol. 1993;  41 914-918
    PubMedGoogle Scholar
  • 31 Terao J, Piskula M, Yao Q. Protective effect of epicatechin, epicatechin gallate, and quercetin on lipid peroxidation in phospholipid bilayers.  Arch Biochem Biophys. 1994;  308 278-284
    CrossrefPubMedGoogle Scholar
  • 32 Vani M, Reddy G P, Reddy G R, Thyagaraju K, Reddana P. Glutathione-S-transferase, superoxide dismutase, xanthine oxidase, catalase, glutathione peroxidase and lipid peroxidation in the liver of exercised rats.  Biochemistry international. 1990;  21 17-26
    PubMedGoogle Scholar
  • 33 Yamashita K, Iizuka Y, Imai T, Namiki M. Sesame seed and its lignans produce marked enhancement of vitamin E activity in rats fed a low α-tocopherol diet.  Lipids. 1995;  30 1019-1028
    PubMedGoogle Scholar

Dr. T. Moritani

Laboratory of Applied Physiology · Graduate School of Human and Environmental Studies

Kyoto University · Sakyo-ku · Kyoto-city · Kyoto 606-8501 · Japan ·

Phone: +81 75 753 6888

Fax: +81 75 753 6888

Email: moritani@virgo.jinkan.kyoto-u.ac.jp

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