Planta Med 2003; 69(7): 610-616
DOI: 10.1055/s-2003-41127
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

In vivo Anti-Inflammatory and Antinociceptive Effects of Liriodendrin Isolated from the Stem Bark of Acanthopanax senticosus

Hyun-Ju Jung1 , Hee-Juhn Park1 , Ryung-Gue Kim2 , Kyoung-Min Shin2 , Joohun Ha3 , Jong-Won Choi4 , Hyoung Ja Kim5 , Yong Sup Lee5 , Kyung-Tae Lee2
  • 1Division of Applied Plant Sciences, Sangji University, Woosan-Dong, Wonju, Korea
  • 2College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Korea
  • 3College of Medicine, Kyung Hee University, Hoegi-Dong, Seoul, Korea
  • 4College of Pharmacy, Kyungsung University, Dayeon-Dong, Pusan, Korea
  • 5Division of Life Sciences, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea
This research was supported by a grant (PF002104-07) from Plant Diversity Research Center of 21st Frontier Research Program funded by Ministry of Science and Technology of Korean government
Further Information

Publication History

Received: September 20, 2002

Accepted: April 26, 2003

Publication Date:
04 August 2003 (online)

Abstract

In the present study, liriodendrin isolated by activity-guided fractionation from the ethyl acetate (EtOAc) extracts of the stem bark of Acanthopanax senticosus, was evaluated for anti-inflammatory and antinociceptive activities. Liriodendrin (5, 10 mg/kg/day, p. o.) significantly inhibited the increase of vascular permeability induced by acetic acid in mice and reduced an acute paw edema induced by carrageenan in rats. When the analgesic activity was measured by the acetic acid-induced writhing test and hot plate test, liriodendrin showed a dose-dependent inhibition in animal models. In addition, syringaresinol, the hydrolysate of liriodendrin, more potently inhibited the LPS-induced production of NO, PGE2 and TNF-α production of macrophages than liriodendrin. Consistent with these observations, the expression level of iNOS and COX-2 enzyme was decreased by syringaresinol in a concentration-dependent manner. These results suggest that the anti-inflammatory and antinociceptive effects of liriodendrin after oral administration were attributable to the in vivo transformation to syringaresinol, which may function as the active constituent.

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Dr. Kyung-Tae Lee

Department of Biochemistry

College of Pharmacy

Kyung-Hee University

Dongdaemun-Ku

Hoegi-Dong 130-701

Seoul

Korea

Phone: +82-2-9610860

Fax: +82-2-9663885

Email: ktlee@khu.ac.kr