Subscribe to RSS
DOI: 10.1055/s-2003-41124
Arbuscular Mycorrhizal Inoculation Enhances Survival Rates and Growth of Micropropagated Plantlets of Echinacea pallida
This work was partially funded by the Agricultural Research Service Specific Cooperative Agreement No. 58-6408-2-0009.Publication History
Received: November 13, 2002
Accepted: April 12, 2003
Publication Date:
04 August 2003 (online)
Abstract
In an attempt to induce positive effects on the acclimatization of in vitro propagated Echinacea pallida, four arbuscular mycorrhizal (AM) fungi, Glomus mosseae, Gigaspora ramisporophora, Scutellospora fulgida and Entrophospora colombiana were selected to aid the soil adaptation process. Fungal inocula affected the survival of E. pallida plantlets ranging from 83 % to 92 %, depending on the AM species and also contrasting with 58 % survival of the non-inoculated plantlets. Growth and development were faster in mycorrhizal treated plantlets than in non-treated ones, especially among those treated with Glomus mossae and Scutellospora fulgida. The presence of well-formed arbuscules and vesicles in Echinacea infested roots was confirmed by microscopic examinations in addition to 90 % success in the survival rate of vigorous plants indicated that mycorrhization is a valuable tool to overcome Echinacea acclimatization shock.
References
- 1 Giles J R, Palat C T, Chien S H, Chang Z G, Kennedy D T. Evaluation of Echinacea for treatment of common colds. Pharmacotherapy. 2000; 20 690-7
- 2 Foster S. Echinacea: Nature’s immune enhancer. Healing arts press Rochester, Vermont; 1991
- 3 Harbage J F. Micropropagation of Echinacea angustifolia, E. pallida, and E. purpurea from stem and seed explants. HortScience. 2001; 36 360-4
- 4 Barea J M, Azcon-Aguilar C. Production of plant growth-regulating substances by the vesicular-arbuscular mycorrhizal fungus Glomus mosseae . Applied Environ Microbiol. 1982; 43 810-3
- 5 Gianinazzi-Pearson V, Dumas-Gaudot E, Gollotte A, Thiri-Alaqui A, Gianinazzi S. Cellular and molecular defence-related root responses to invasion by arbuscular mycorrhizal fungi. New Phytologist. 1996; 133 45-57
- 6 Codignola A, Verotta A, Spanu P, Maffei M, Scannerini S, Bonfante-Fasolo P. Cell wall bound-phenols in roots of mycorrhizal plants. New Phytologist. 1989; 112 221-8
- 7 Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962; 15 473-7
- 8 Sieverding E. Vesicular-arbuscular mycorrhiza management in tropical agrosystems. Deutsche Gesellschaft für technische Zusammenarbeit (GTZ) GmbH Eschborn, Germany; 1991;: 331
- 9 Philips J M, Hayman D S. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. British Mycology Trans. 1970; 5 158-61
- 10 McGonigle T P, Miller M H, Evans D G, Fairchild G L, Swan J A. A new method, which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytologist. 1990; 115 495-501
- 11 Perry N B, Burgess E J, Glennie V L. Echinacea Standardization: Analytical methods for phenolic compounds and typical levels in medicinal species. J Agri Food Chem. 2001; 49 1702-6
-
12 SPSS 9.0 for W indows. SPSS Copywright Inc Chicago, Illinois; 1998
Prof. Rita Moraes
National Center for Natural Products Research
Research Institute of Pharmaceutical Sciences
School of Pharmacy
The University of Mississippi
University
MS 38677
USA
Phone: +1-662-915-1147
Fax: +1-662-915-1062
Email: rmoraes@olemiss.edu.