Induction of Apoptosis and G₂/M Cell Cycle Arrest by Gleditsioside E from Gleditsia sinensis in HL-60 Cells

Abstract

Gleditsioside E, a triterpene saponin isolated from Gleditsia sinensis, showed significant cytotoxicity against Bel-7402, BGC-823, HeLa, HL-60 and MCF-7 cell lines. The results of flow cytometry with annexin V-FITC/PI double staining proved that gleditsioside E mainly induced early apoptosis in HL-60 cells. Gleditsioside E treatment resulted in a prominent increase of the G₂/M population in HL-60 cells, and an accumulation of the sub-G₁ (hypoploid) peak was observed.

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