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Planta Med 2001; 67(9): 839-842
DOI: 10.1055/s-2001-18853
Original Paper
Biochemistry, Physiology, in vitro-cultures
© Georg Thieme Verlag Stuttgart · New York

Formation of Protoberberine-Type Alkaloids by the Tubers of Somatic Embryo-Derived Plants of Corydalis yanhusuo

Ya-Lin Lee1 , Abhay P. Sagare2 , Chen-Yue Lee2 , Hai-Tung Feng3 , Yen-Chen Ko3 , Jei-Fu Shaw4 , Hsin-Sheng Tsay2
  • 1 FooYin Institute of Technology, Department of Biotechnology, Taliou, Kaohsiung County, Taiwan
  • 2 Department of Agronomy, Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan
  • 3 Pesticide Chemistry Department, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Wufeng, Taichung, Taiwan
  • 4 Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan
Further Information

Publication History

November 9, 2000

March 24, 2001

Publication Date:
06 December 2001 (online)

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Abstract

The effects of 0.5 - 5 mg/l abscisic acid [ABA], 0.5 - 10 mg/l (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol [paclobutrazol] and 0.5 - 2 mg/l α-cyclopropyl-α-(4-methoxyphenyl)-5-pyrimidinemethanol [ancymidol], 0.5 - 5 mg/l gibberellic acid [GA3] and 15 - 100 mg/l polyethylene glycol [PEG] 4000 supplemented in half-strength Murashige and Skoog’s (MS) medium on the production of the two major protoberberine-type alkaloids (D,L-tetrahydropalmatine and D-corydaline) by the tubers of somatic embryo-derived plants of Corydalis yanhusuo were examined. Somatic embryo derived plants were also maintained for 6 months on half-strength MS medium containing 0.1 mg/l GA3 or 0.5 mg/l paclobutrazol. The alkaloid contents were determined by high performance liquid chromatography (HPLC). The analysis revealed that the contents of D,L-tetrahydropalmatine and D-corydaline in the tubers of somatic embryo-derived plants were higher than the marketed crude drug and varied with growth regulator/PEG-4000 treatment and the age of the plant.

Key words

D-Corydaline - Corydalis yanhusuo - Fumariaceae - HPLC - protoberberine alkaloids - D,L-tetrahydropalmatine - tissue culture - yanhusuo

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Dr. Hsin-Sheng Tsay

Department of Agronomy

Taiwan Agricultural Research Institute

Wu-feng

Taichung 41301

Taiwan

Republic of China

Email: hstsay@wufeng.tari.gov.tw

Fax: +886-4-23338162

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