Abstract
Rehydration of powdered tissues of Podophyllum peltatum L. prior to extraction with an organic solvent allows endogenous β-glucosidases to
hydrolyze lignan 4-O -β-D-glucosides in situ and increase the yield of podophyllotoxin. Aqueous extraction of rhizomes and leaves
of P. peltatum yielded 4- to 10-fold greater quantities of podophyllotoxin than the traditional
ethanolic extraction. Most significantly, leaves were shown to contain over 52 mg
of podophyllotoxin per g of dry weight (5.2 %), exceeding levels previously reported
from any source. These results point to the use of leaves harvested from cultivated
P. peltatum as an attractive alternative to the destructive collection of natural populations.
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Dr. Franck E. Dayan
USDA, ARS, NPURU
National Center for Natural Products Research
University
MS 38677
USA
Email: fdayan@olemiss.edu
Fax: +1 662 915-1035