Environmental Homeopathy: Homeopathic Potencies Regulate the Growth and Toxicity of Raphidiopsis raciborskii (cyanobacteria) and Can be Tracked Physico-Chemically. Part 2: Physico-chemical Results

Abstract

Introduction The control of cyanobacterial toxicity and growth by homeopathic potencies was described in Part 1 of this two-part report. Here, a parallel approach characterized the physico-chemical features of the potencies used and the liquid media treated with them, correlating these results with their respective biological effects.

Objectives Our objective was to establish if physico-chemical parameters can track homeopathic potencies in seawater or artificial seawater medium (ASM)-1 and to discover whether these parameters correlate with previously described biological effects.

Method Artemia franciscana (brine shrimp) cysts were cultivated in seawater challenged with Raphidiopsis raciborskii extract and treated with different homeopathic potencies chosen from a screening process. Cultures of R. raciborskii maintained in ASM-1 were also treated with previously screened homeopathic potencies, and their growth was monitored as a function of time. The physico-chemical properties of the treated media (seawater or ASM-1) were evaluated by their interaction with solvatochromic dyes and changes in pH, conductivity and temperature.

Results Coumarin 7 was found to be a marker for Nitric acidum 6cH and Isotherapic (R. raciborskii extract) 200cH in seawater (analysis of variance [ANOVA], p = 0.0015). Nile red was found to be a marker for Nitric acidum 200cH and Mercurius solubilis 30cH in ASM-1 (ANOVA, p ≤ 0.001). An increase in pH of ASM-1 and endothermic effects were observed after these treatments (two-way ANOVA, p = 0.0001). Seawater and ASM-1 to which potencies had been added were also subjected to a constant unidirectional 2,400 Gauss static magnetic field and found to have enhanced effects on the solvatochromic dyes tested.

Conclusion Homeopathic potencies were specifically traceable in aqueous media using solvatochromic dyes, especially when the samples were subjected to a magnetic field. Results from monitoring other physical parameters, such as pH and temperature, were less specific in relation to potency tracking. However, potency-induced endothermic effects might provide valuable thermodynamic data relating to the nature of potencies.

Keywords

water - solvatochromic dyes - physico-chemical markers - homeopathy

Author Attributions

S.N.M. was the main researcher, Ph.D. student, involved in all experimental procedures.

A.A.G.P. was responsible for experimental procedures—toxicity and solvatochromic dyes tests.

R.A.S. was responsible for experimental procedures—gene expression tests.

I.B.S. was responsible for experimental procedures—solvatochromic dyes tests.

A.L.T. was responsible for the discussion of results on physicochemical parameters.

S.J.C. was responsible for experimental design with solvatochromic dyes and the discussion of results.

J.S.Y. was the co-adviser and oversaw cyanobacteria standards and contributed to the discussion about results.

L.V.B. was the main adviser and was responsible for the coordination of all steps of the study and contributed to the discussion about results.

Publikationsverlauf

Eingereicht: 25. August 2023

Angenommen: 14. Dezember 2023

Artikel online veröffentlicht:
06. Mai 2024

• References

• 1 Mohammad SN, Pinto AAG, da Silva RA. et al Eco-homeopathy: homeopathic potencies regulate the growth and toxicity of Raphidiopsis raciborskii (cyanobacteria) and can be tracked by physico-chemically. Part 1: Biological models. Homeopathy 2024: online ahead of print
• 2 Tan J, MacRae TH. Stress tolerance in diapausing embryos of Artemia franciscana is dependent on heat shock factor 1 (HSF1). PLoS One 2018; 13: e0200153
• 3 King AM, Toxopeus J, MacRae TH. Functional differentiation of small heat shock proteins in diapause-destined Artemia embryos. FEBS J 2013; 280: 4761-4772
• 4 MacRae TH. Stress tolerance during diapause and quiescence of the brine shrimp, Artemia. Cell Stress Chaperones 2016; 21: 9-18
• 5 Han J, Park Y, Shin HH. et al. Effects of dinoflagellate Gymnodinium catenatum on swimming behavior and expression of heat shock protein (hsp) genes in the brine shrimp Artemia franciscana . Harmful Algae 2021; 110: 102146
• 6 Gbotsyo YA, Rowarth NM, Weir LK, MacRae TH. Short-term cold stress and heat shock proteins in the crustacean Artemia franciscana . Cell Stress Chaperones 2020; 25: 1083-1097
• 7 Beristain P, Gajardo G, Bossier P. Species-specific RFLP pattern in the Heat Shock Protein26 gene (Hsp26): a single-locus tool for species identification and experimental testing of habitat-induced isolation in the New World Artemia species. Mol Ecol Resour 2010; 10: 229-231
• 8 Qiu Z, Bossier P, Wang X, Bojikova-Fournier S, MacRae TH. Diversity, structure, and expression of the gene for p26, a small heat shock protein from Artemia. Genomics 2006; 88: 230-240
• 9 Calderwood SK, Mambula SS, Gray Jr PJ, Theriault JR. Extracellular heat shock proteins in cell signaling. FEBS Lett 2007; 581: 3689-3694
• 10 Carver JA, Rekas A, Thorn DC, Wilson MR. Small heat-shock proteins and clusterin: intra- and extracellular molecular chaperones with a common mechanism of action and function?. IUBMB Life 2003; 55: 661-668
• 11 Gorham PR, McLachlan J, Hammer UT, Kim WK. Isolation and culture of toxic strains of Anabaena flos aquae (Lyngb.) de Bréd. Verh. Internat. Verein. Limnol. 1964; 15: 796-804
• 12 Pinto AAG, Nagai MYO, Coimbra EN. et al. Bioresilience to mercury chloride of the brine shrimp Artemia salina after treatment with homeopathic Mercurius corrosivus . Homeopathy 2021; 110: 244-255
• 13 Coimbra Melo EN. Efeito protetor do isoterápico sobre a eclosão de cistos de Artemia salina intoxicadas com arseniato de sódio [PhD thesis]. São Paulo: Graduation Program on Environmental and Experimental Pathology, Universidade Paulista—UNIP; 2020. Accessed November 5, 2023 at: https://repositorio.unip.br/programa-de-pos-graduacao-stricto-sensu-em-patologia-ambiental-e-experimental/efeito-protetor-do-isoterapico-sobre-a-eclosao-de-cistos-de-artemia-salina-intoxicadas-com-arseniato-de-sodio/
• 14 Nagai MO, Pinto AAG, von Ancken AC. et al. Exposure of Artemia salina to glyphosate and bioremediation by isotherapy. Proceedings of the XXXIV GIRI meeting. Int J High Dilution Res 2022; 21: 13
• 15 Calabrese EJ, Giordano J. Ultra low doses and biological amplification: approaching Avogadro's number. Pharmacol Res 2021; 170: 105738
• 16 López-Otín C, Kroemer G. Hallmarks of health. Cell 2021; 184: 33-63
• 17 Ullman D. Exploring possible mechanism of hormesis and homeopathy in the light of nanopharmacology and ultra-high dilutions. Dose Response 2021; 19: 15 593258211022983
• 18 Nagai MYDO, Mohammad SN, Pinto AAG. et al. Highly diluted glyphosate mitigates its effects on Artemia salina: physicochemical implications. Int J Mol Sci 2023; 24: 9478
• 19 Bonamin LV, Pedro RRP, Mota HMG. et al. Characterization of Antimonium crudum activity using solvatochromic dyes. Homeopathy 2020; 109: 79-86
• 20 Cartwright SJ. Solvatochromic dyes detect the presence of homeopathic potencies. Homeopathy 2016; 105: 55-65
• 21 Cartwright SJ. Interaction of homeopathic potencies with the water soluble solvatochromic dye bis-dimethylaminofuchsone. Part 1: pH studies. Homeopathy 2017; 106: 37-46
• 22 Aparicio ACC, de Oliveira LHS, Silva JS. et al. Interaction between solvatochromic dyes and water sampled from a natural source treated with high dilutions of phosphorus. Homeopathy 2020; 109: 126-132
• 23 Cartwright SJ. Degree of response to homeopathic potencies correlates with dipole moment size in molecular detectors: implications for understanding the fundamental nature of serially diluted and succussed solutions. Homeopathy 2018; 107: 19-31
• 24 Cartwright SJ. Homeopathic potencies may possess an electric field(-like) component: evidence from the use of encapsulated solvatochromic dyes. Homeopathy 2020; 109: 14-22
• 25 Cartwright SJ. Immobilization of solvatochromic dyes on transparent cellulose films: an improved method for the study of homeopathic potencies. Homeopathy 2023; 112: 125-134
• 26 Pinto SAG, Aparicio ACC, Souza JS, Suffredini IB, Cartwright SJ, Bonamin LV. Characterization of physicochemical markers for homeopathic medicines and biological supernatant samples. Proceedings of the XXXIV GIRI meeting. Int. J. High Dilution Res 2022; 21: 04
• 27 Del Giudice E, Preparata G, Vitiello G. Water as a free electric dipole laser. Phys Rev Lett 1988; 61: 1085-1088
• 28 Del Giudice E, Preparata G, Fleischmann M. QED coherence and electrolyte solutions. J Electroanalyt Chem (Lausanne) 2000; 482: 110-116
• 29 Elia V, Napoli E. Dissipative structures in extremely diluted solutions of homeopathic medicines. A molecular model based on physicochemical and gravimetric evidence. Int J Des Nat. 2010; 5: 39-48
• 30 Yinnon TA, Liu ZQ. Domains formation mediated by electromagnetic fields in very dilute aqueous solutions: 3. Quantum electrodynamic analyses of experimental data on solutions of weak electrolytes and non-electrolytes. Water 2015; 7: 70-95
• 31 Mahata CR. Dielectric dispersion studies of some potentised homeopathic medicines reveal structured vehicle. Homeopathy 2013; 102: 262-267
• 32 Benveniste J. Further biological effects induced by ultra-high dilutions. Inhibitions by a magnetic field. In: Endler PC, Shulte J. eds. Ultra High Diluiton. Dordrecht: Kluwer Academic Publishers; 1994: 35-38
• 33 Salles N, Frana S, Souza MF. et al. Solvatochromic dyes as a tool for tracking homeopathic complex activity in water reservoirs of a spring park in Brazil: physicochemical implications. Proceedings of the XXXVI GIRI meeting, October 20-22, 2023. Farmington, CT. USA. Int J High Dilution Res 2023; 22: 48
• 34 Holandino C, Harduim R, Veiga VF, Zacharias CR. Modeling physical-chemical properties of high dilutions: an electrical conductivity study. Int J High Dilution Res 2008; 7: 165-173
• 35 Verdel N, Jerman I, Krasovec R, Bukovec P, Zupancic M. Possible time-dependent effect of ions and hydrophilic surfaces on the electrical conductivity of aqueous solutions. Int J Mol Sci 2012; 13: 4048-4068
• 36 Paul BK, Kar S, Bandyopadhyay P. et al. Significant enhancement of dielectric and conducting properties of electroactive polymer polyvinylidene fluoride films: an innovative use of Ferrum metallicum at different concentrations. Indian J Res Homoeopathy 2016; 10: 52-58
• 37 Jerman I, Ogrizek L, Jan L, Krapez VP. Physico-chemical and UV/VIS measurements of UHD solutions of water, potentized water and different potencies of organic substances. Proceedings of the XXXV GIRI Meeting. Berlin, Germany. Int J High Dilution Res 2022; 21: 15-16
• 38 Ciavatta L, Elia V, Napoli E, Niccoli M. New physico-chemical properties of extremely diluted solutions. Electromotive force measurements of galvanic cells sensible to the activity of NaCl at 25 degrees C. J Solution Chem 2008; 37: 1037-1049
• 39 Elia V, Elia L, Napoli E, Niccoli M. Conductometric and calorimetric studies of serially diluted and agitated solutions: the dependence of intensive parameters on volume. Int J Ecodynamics 2006; 1: 361-372
• 40 Holandino C, Oliveira AP, Homsani F. et al. Structural and thermal analyses of zinc and lactose in homeopathic triturated systems. Homeopathy 2017; 106: 160-170
• 41 Fontes C, Oliveira AP, Batista JVC. et al. Physicochemical properties of zinc and lactose in solid mixtures: influence of trituration process. Homeopathy 2022; 111: 164-175
• 42 Silva RDS, Chia MA, Barbosa VV. Synergistic effects of temperature and nutrients on growth and saxitoxin content of the cyanobacterium Raphidiopsis raciborskii . J Appl Phycol 2022; 34: 941-952
• 43 Werner VR, Tucci A, da Silva LM. et al. Morphological, ecological, and toxicological aspects of Raphidiopsis raciborskii (Cyanobacteria) in a eutrophic urban subtropical lake in southern Brazil. Iheringia. Série Botânica, Porto Alegre. 2020; 75: e2020018
• 44 Galvanese EF, Padial AA, Aubriot L. Acclimation at high temperatures increases the ability of Raphidiopsis raciborskii (Cyanobacteria) to withstand phosphate deficiency and reveals distinct strain responses. Eur J Phycol 2019; 54: 359-368
• 45 Sinclair JR. Importance of a One Health approach in advancing global health security and the sustainable development goals. Rev Sci Tech 2019; 38: 145-154
• 46 Lubroth J. FAO and the One Health approach. Curr Top Microbiol Immunol 2013; 366: 65-72