Therapeutic effects of Acer palmatum Thumb. leaf extract (KIOM-2015E) in a mouse Benzalkonium Chloride-induced dry eye model

We confirmed the protective effects of Acer palmatum Thumb. leaf extract against the degeneration of rat retinal ganglion cells after ischemia/reperfusion (I/R) induced by midbrain cerebral artery occlusion (MCAO) in a previous study. Referring to the results of that, in this study, we determined the effects of two kinds of

A. palmatum Thumb. leaf extract (KIOM-2015E, hot water extract KIOM-2015EW and 25% ethanol extract KIOM-2015EE) in a benzalkonium chloride (BAC)-induced dry eye mouse model. Dry eye was induced by 0.2% BAC for 2 weeks, followed by treatment three times (eye drop) or once (oral administration) daily with KIOM-2015E for 2 weeks. Treatment with both KIOM-2015EE and KIOM-2015EW resulted in a marked increase in tear volume production for the 4 days of treatment. The Lissamine Green staining score, TUNEL- positive cells and inflammatory index were significantly decreased after 2 weeks. Topical KIOM-2015EE administration exhibited greater improvement in decreasing the ocular surface staining scores, inflammation, dead cells and increasing tear production in a dose-dependent manner compared with the other groups. Furthermore, KIOM-2015E significantly reduced the phosphorylation of NF-κB, which was activated in the BAC-treated cornea. Topical administration was much more effective than oral administration for KIOM-2015E and KIOM-2015EE was more effective than KIOM-2015EW. Application of KIOM-2015E resulted in clinical improvement, including inhibiting the inflammatory response and alleviating signs of dry eye. Taken together, these results indicate that KIOM-2015E has potential as a therapeutic agent for the clinical treatment of dry eye.

 

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Artikel online veröffentlicht:
16. November 2023