Antioxidant, and Anti-α-glucosidase Activities of Various Solvent Extracts and Major Bioactive Components from Ampelopsis japonica

The dried root of Ampelopsis japonica (Thunb.) Makino (A. japonica) is a traditional medicine used to treat fever, pain and wound healing. It exhibits anti-inflammatory, antitumour, anti-tyrosinase and anti- melanogenic activities. In this congress, we used different solvent extracts from the root of A. japonica to determine their antioxidant activity. Acetone extract showed relatively strong antioxidant properties by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), superoxide radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. In addition, these extracts also showed significant α-glucosidase inhibitory activities. Acetone extract significantly inhibited α-glucosidase with an IC₅₀ value of 8.30±0.78 μg/mL. Using HPLC analysis and comparison with the chemical composition of various solvent extracts, we isolated seven active compounds and assessed their antioxidant and anti-α-glucosidase activities. Catechin (1), gallic acid (2), kaempferol (3), quercetin (4), resveratrol (6), and epicatechin (7) were the main antioxidant components in the root of A. japonica. According to the results of DPPH, ABTS and superoxide radical scavenging assays, these isolates showed stronger antioxidant capacity than butylated hydroxytoluene (BHT). Moreover, 1, 3, 4, euscaphic acid (5), 6 and 7 also expressed stronger anti-α-glucosidase activity than the positive control acarbose. This study indicates that A. japonica and its active extracts and components may be a promising source of natural antioxidants and α-glucosidase inhibitors.

Publication History

Article published online:
16 November 2023