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Allgemeine Homöopathische Zeitung 2017; 262(04): 15-21
DOI: 10.1055/s-0043-107826
Spektrum
Karl F. Haug Verlag in Georg Thieme Verlag KG Stuttgart · New York

Die Erforschung molekularer Aspekte homöopathischer Arzneiwirkungen

Teil 2: Weitere experimentelle Unterstützung zugunsten der Genregulationshypothese
Anisur Rahman Khuda-Bukhsh  
Further Information

Publication History

Publication Date:
28 August 2017 (online)

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Zusammenfassung

In den letzten Jahren wurden mithilfe modernster Elektronenmikroskopie durch mehrere Autoren Nanopartikel von den Ausgangssubstanzen von Arzneien nachgewiesen. Während man annimmt, dass diese Nanopartikel von Arzneien in Hochpotenzen von der homöopathischen Potenzierung herrühren, haben wir mit einer wissenschaftlich anerkannten Methode Nanokapseln aus den Extrakten homöopathischer Urtinkturen und ihren biologisch aktiven Inhaltsstoffen produziert, wofür wir das nicht-toxische, biologisch abbaubare Polymer Poly(lactid-co-glycolid) nutzten sowie Mechanismus und Wirkungspfade ihrer biologischen Aktivität untersuchten.

Summary

In recent years, nanoparticles of original drug substances have been detected by several authors using advanced electron microscopy. While these nanoparticles of drugs in high potencies are believed to be derived during the process of homeopathic succussion/dynamization/potentiation, we produced with a scientifically accepted method nanocapsules of homeopathic mother tincture extracts and their biologically active ingredients using non-toxic biodegradable polymer Poly(lactid-co-glycolid) and studied the mechanism and pathways of their biological action.

Schlüsselwörter

Genregulationshypothese - Nanopartikel

Keywords

Gene regulatory hypothesis - nanoparticles

Anmerkungen

1 Übersetzung: Thomas Mickler


Bibliografie

DOI https://doi.org/10.1055/s-0043-107826

AHZ 2017; 262: 15–21

© Georg Thieme Verlag KG Stuttgart · New York

ISSN 1438-2563

 

  • Literatur

  • 1 Bhattacharyya SS, Paul S, Dutta S. et al. Anti-oncogenic potentials of a plant coumarin (7-hydroxy coumarin) against DMBA induced skin papilloma in mice: The possible role of several key signal proteins. J Chin Integr Med 2010; 8: 645-654
    CrossrefGoogle Scholar
  • 2 Bhattacharyya SS, Paul S, Khuda-Bukhsh AR. Encapsulated plant extract (gelsemium sempervirens) poly (lactide-co-glycolide) nanoparticles enhance cellular uptake and increases bioactivity in vitro. Exp Biol Med (Maywood) 2010; 235 (04) 678-688 DOI: 10.1258/ebm.2010.009338
    CrossrefGoogle Scholar
  • 3 Bhattacharyya SS, Paul S, De A. et al. Poly (lactide-co-glycolide) acid nanoencapsulation of a synthetic coumarin: Cytotoxicity and bio-distribution in mice, in cancer cell line and interaction with calf thymus DNA as target. Toxicol Appl Pharmacol 2011; 253: 270-281 DOI: 10.1016/j.taap.2011.04.010
    CrossrefGoogle Scholar
  • 4 Bhattacharyya SS, Das J, Das J, Das S. et al. Rapid green synthesis of silver nanoparticles from silver nitrate by a homeopathic mother tincture phytolacca decandra. J Chin Integr Med 2012; 10: 546-554
    CrossrefGoogle Scholar
  • 5 Bishayee K, Sikdar S, Khuda-Bukhsh AR. Ultra-highly diluted gonolobus condurango extract inhibits histone de-acetylase2 activity in cervix cancer cells in vitro: Evidence of epigenetic modification in cell cycle arrest. J Pharmacopuncture 2013; 16: 7-13
    CrossrefPubMedGoogle Scholar
  • 6 Biswas R, Mandal SK, Dutta S. et al. Thujone rich fraction of thuja occidentalis demonstrates major anti-cancer potentials: Evidences from in vitro studies on A375 cells. eCAM 2011 DOI: DOI: 10.1093/ecam/neq042
    Google Scholar
  • 7 Chakraborty D, Ghosh S, Bishayee K. et al. Anti-hyperglycemic drug Gymnema sylvestre also shows anti-cancer potentials in human melanoma A375 cells via ROS generation and mitochondria-dependent caspase pathway. Integrative Cancer Therapies 2013; 12: 433-441 DOI: 10.1177/1534735413485419
    CrossrefGoogle Scholar
  • 8 Chakraborty D, Sikdar S, Bishayee K. et al. Combination of helonias dioica extract and metformin improves insulin responsiveness by activating PI3K/AKT pathway signaling in obese hyperglycemic mice. J Chin Integr Med Springer [in press]
  • 9 Das J, Das S, Samadder A. et al. Poly(lactide-co-glycolide) encapsulated extract of phytolacca decandra demonstrates better intervention against induced lung adenocarcinoma in mice and on A549 cells. Eur J Pharm Sci 2012; 29: 313-324 DOI: 10.1016/j.ejps.2012.06.018
    Google Scholar
  • 10 Das S, Das J, Paul A. et al. Apigenin, a bioactive flavonoid from Lycopodium clavatum, stimulates nucleotide excision repair genes to protect skin keratinocytes from ultraviolet B-induced reactive oxygen species and DNA damage. J Acupunct Meridian Stud 2013; 6: 252-262
    CrossrefGoogle Scholar
  • 11 Das S, Das J, Samadder A. et al. Biosynthesized silver nanoparticles by ethanolic extracts of phytolacca decandra, gelsemium sempervirens, hydrastis canadensis and thuja occidentalis induce differential cytotoxicity through G2/M arrest in A375 cells. Colloids Surf B Biointerfaces 2013; 101: 325-336
    CrossrefGoogle Scholar
  • 12 Das S, Das J, Samadder A. et al. Efficacy of PLGA-loaded apigenin nanoparticles in Benzo[a]pyrene and ultraviolet-B induced skin cancer of mice: Mitochondria mediated apoptotic signaling cascades. Food Chem Toxicol 2013; Oct 9. DOI: pii: S0278-6915(13)00675-3 DOI: 10.1016/j.fct.2013.09.037.
    Google Scholar
  • 13 Das S, Das J, Samadder A. et al. Strategic formulation of apigenin-loaded PLGA nanoparticles for intracellular trafficking, DNA targeting and improved therapeutic effects in skin melanoma in vitro. Toxicol Lett 2013; 223: 124-138 DOI: 10.1016/j.toxlet.2013.09.012
    CrossrefGoogle Scholar
  • 14 Khuda-Bukhsh AR. Alteration of X-ray effects by homeopathic drugs: A new approach in radio-protection. In: Manna GK, Roy SC. eds. Perspectives in Cytology and Genetics. Vol.8. Calcutta: All India Congress of Cytology and Genetics; 1995: 649-663
    Google Scholar
  • 15 Khuda-Bukhsh AR. Potentized homeopathic drugs act through regulation of gene expression: A hypothesis to explain their mechanism and pathways of action in vivo. Comp Ther Med 1997; 5: 43-46
    CrossrefGoogle Scholar
  • 16 Khuda-Bukhsh AR, Bhattacharyya SS, Paul S, Boujedaini N. Polymeric nanoparticle encapsulation of a naturally occurring plant scopoletin and its effects on human melanoma cell A375. J Chin Intgr Med 2010; 8: 853-862
    Google Scholar
  • 17 Mondal J, Panigrahi AK, Khuda-Bukhsh AR. Physico-chemical and ultra-structural characterizations of PLGA-loaded nanoparticles of Boldine and their efficacy in ameliorating cisplatin induced hepatotoxicity in normal liver cells in vitro. Journal of Innovations in Pharmaceuticals and Biological Sciences 2015; 2: 506-521
    Google Scholar
  • 18 Mondal J, Panigrahi AK, Khuda-Bukhsh AR. Poly(lactic-co-gycolic acid)-loaded boldine nanoparticles alleviates cisplatin induced hepatotoxicity in normal liver cells in vitro via DNA targeting: Drug-DNA interaction. International Journal of Therapeutic Applications 2015; 29: 24-31
    Google Scholar
  • 19 Paul S, Bhattacharyya SS, Boujedaini N, Khuda-Bukhsh AR. Anticancer potentials of root extract of polygala senega and its PLGA nanoparticles-encapsulated form. Evid Based Complement Alternat Med 2011. DOI: DOI: 10.1155/2011/517204
    Google Scholar
  • 20 Paul A, Das J, Das S. et al. PLGA nano-encapsulation of chelidonine enhances the ameliorative potential against cadmium induced oxidative damage and hepatic injury in mice. Environ Toxicol Pharmacol 2013; 36: 937-947
    CrossrefGoogle Scholar
  • 21 Paul A, Das S, Das J. et al. Cytotoxicity and apoptotic signaling cascade induced by chelidonine-loaded PLGA nanoparticles in HepG2 cells in vitro and bioavailability of nano-chelidonine in mice in vivo. Toxicol Lett 2013; 222: 10-22
    CrossrefGoogle Scholar
  • 22 Saha SK, Roy S, Khuda-Bukhsh AR. Evidence in support of gene regulatory hypothesis: Gene expression profiling manifests homeopathy effect as more than placebo. Int J High Dilution Res 2013; 12: 162-167
    Google Scholar
  • 23 Saha S, Khuda-Bukhsh AR. Berberine alters epigenetic modifications, disrupts microtubule network, and modulates HPV-18E6-E7 oncoproteins by targeting p53 in cervical cancer cell HeLa: A mechanistic study including molecular docking. European Journal of Pharmacology 2014; 244: 132-146
    Google Scholar
  • 24 Saha SK, Roy S, Khuda-Bukhsh AR. Ultra-highly diluted plant extracts of hydrastis canadensis and marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. J Integr Med 2015; 13: 400-411 DOI: 10.1016/S2095-4964(15)60201-1
    CrossrefGoogle Scholar
  • 25 Samadder A, Chakraborty D, De A. et al. Possible signaling cascades involved in attenuation of alloxan-induced oxidative stress and hyperglycemia in mice by ethanolic extract of Syzygium jambolanum: Drug-DNA interaction with calf thymus DNA as target. Eur J Pharm Sci 2011; 9: 207-217 DOI: 10.1016/j.ejps.2011.07.012
    Google Scholar
  • 26 Samadder A, Das J, Das S. et al. Dihydroxy-isosteviol methyl ester of Pulsatilla nigricans extract reduces arsenic-induced DNA damage in testis cells of male mice: Its toxicity, drug-DNA interaction and signaling cascades. J Chin Integr Med 2012; 10: 1433-1442 DOI: 10.3736/jcim20121216
    CrossrefGoogle Scholar
  • 27 Samadder A, Das J, Das S, Khuda-Bukhsh AR. Dihydroxy-isosteviol-methyl-ester, an active biological component of pulsatilla nigricans, reduces arsenic induced cellular dysfunction in testis of male mice. Environ Toxicol Pharmacol 2012; 34: 743-752 DOI: 10.1016/j.etap.2012.09.013
    CrossrefGoogle Scholar
  • 28 Samadder A, Das S, Das J. et al. Ameliorative effects of syzygium jambolanum extract and its poly(lactic-co-glycolic) acid nano-encapsulated form on arsenic induced hypoglycemic stress: A multiparametric evaluation. J Acupunct Meridian Stud 2012; 5: 310-318
    CrossrefGoogle Scholar
  • 29 Sikdar S, Khuda-Bukhsh AR. Condurango 30C induces epigenetic modification of lung cancer specific tumour suppressor genes via demethylation. Forschende Komplementärmedizin/Research in Complementary Medicine 2015; 22: 172-179 DOI: 10.1159/000433485
    CrossrefGoogle Scholar