Development of an Enzyme-Linked Immunosorbent Assay for Determination Of Miroestrol Using an Anti-miroestrol Monoclonal Antibody

Tharita Kitisripanya; Supaluk Krittanai; Orapin Udomsin; Kamonthip Jutathis; Jukrapun Komaikul; Thaweesak Juengwatanatrakul; Seiichi Sakamoto; Hiroyuki Tanaka; Satoshi Morimoto; Waraporn Putalun

doi:10.1055/s-0043-102689

Planta Medica, Table of Contents

Planta Med 2017; 83(10): 855-861
DOI: 10.1055/s-0043-102689

Natural Product Chemistry and Analytical Studies

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Development of an Enzyme-Linked Immunosorbent Assay for Determination Of Miroestrol Using an Anti-miroestrol Monoclonal Antibody

Tharita Kitisripanya

¹Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

²Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University – Khon Kaen University, Khon Kaen, Thailand

,

Supaluk Krittanai

¹Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

²Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University – Khon Kaen University, Khon Kaen, Thailand

,

Orapin Udomsin

¹Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

²Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University – Khon Kaen University, Khon Kaen, Thailand

,

Kamonthip Jutathis

³Regional Medical Sciences Center 7, Department of Medical Sciences, Khon Kaen, Thailand

,

Jukrapun Komaikul

¹Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

²Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University – Khon Kaen University, Khon Kaen, Thailand

,

Thaweesak Juengwatanatrakul

⁴Faculty of Pharmaceutical Sciences, UbonRatchathani University, Ubon Ratchathani, Thailand

,

Seiichi Sakamoto

⁵Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan

,

Hiroyuki Tanaka

⁵Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan

,

Satoshi Morimoto

⁵Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan

,

Waraporn Putalun

¹Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

²Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University – Khon Kaen University, Khon Kaen, Thailand

› Author Affiliations

Abstract

Miroestrol is a chromene with potent estrogenic activity present in Pueraria candollei, commonly known as White Kwao Krua. Although this compound is only present in low amounts in the plant, it plays an important role in the estrogenic action of P. candollei products. As a tool for further studies about the efficacy and safety of P. candollei as a phytoestrogenic supplement, we generated a novel monoclonal antibody against miroestrol. This anti-miroestrol monoclonal antibody was used to develop an immunoassay for the determination of miroestrol content, which can be used for quality control purposes of P. candollei. The developed ELISA against miroestrol has a calibration range of 10–780 ng/mL miroestrol, a limit of detection of 3.5 ng/mL, and a limit of quantitation of 12.2 ng/mL. According to the validation analysis, the established ELISA is precise, accurate, specific, and sensitive for miroestrol detection in plants. Furthermore, the anti-miroestrol monoclonal antibody was used to prepare an immunoaffinity column for the isolation of miroestrol from the tuberous root of P. candollei. The column provides a simple procedure for miroestrol isolation, with a capacity of 3.91 µg of miroestrol per 1 mL of immunogel.

Key words

miroestrol - monoclonal antibody - enzyme-linked immunosorbent assay (ELISA) - immunoaffinity column - White Kwao Krua - Pueraria candollei - Fabaceae

Full Text

References

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