Maslinic Acid Derivative Nanoemulsion: Physicochemical Characterization, Antimicrobial Activity and Three-Dimensional (3D) Reconstructed Human Epidermal Model
          Screening

I Z Magyari-Pavel; L Vlaia; E-A Moacă; L Barbu; D Muntean; A Cioca; S Avram; D Minda; D M Muntean; R Csuk; C A Dehelean; C Danciu

doi:10.1055/s-0042-1759355

Planta Medica, Table of Contents

Planta Med 2022; 88(15): 1574
DOI: 10.1055/s-0042-1759355

Poster Session II

Maslinic Acid Derivative Nanoemulsion: Physicochemical Characterization, Antimicrobial Activity and Three-Dimensional (3D) Reconstructed Human Epidermal Model Screening

I Z Magyari-Pavel

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

L Vlaia

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

E-A Moacă

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

L Barbu

²Faculty of Biology & Geology, “Babes-Bolyai” University, 5 – 7 Clinicilor Street, RO-400006 Cluj-Napoca, Romania; National Institute for R&D of Isotopic and Molecular Technologies, 67 – 103 Donat Street, RO-400293 Cluj-Napoca, Romania

,

D Muntean

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

A Cioca

³Department of Pathology, Regina Maria Health Network, Calea Aradului, No. 113, 300645 Timişoara, Romania

,

S Avram

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

D Minda

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

D M Muntean

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

R Csuk

⁴Martin-Luther University Halle-Wittenberg, Kurt-Mothes-Str. 2, D-06120 Halle (Saale), Germany

,

C A Dehelean

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

,

C Danciu∗

¹“Victor Babeș” University of Medicine and Pharmacy, 2, Eftimie Murgu Square, 300041, Timișoara, Romania

› Author Affiliations

Abstract

Full Text

Background: Maslinic acid belongs to the class of pentacyclic triterpene and has been reported to possess various therapeutic effects, including anti-inflammatory, antimicrobial and antitumor properties. Furthermore, derivatives of maslinic acid have been indicated to elicit improved biological activity compared to the parent compound.

Aim: The present study focuses on the formulation and characterization of a maslinic acid derivative (EM2) nanoemulsion followed by evaluation of the antimicrobial activity and the possible skin irritation effect induced on the three-dimensional (3D) reconstructed human epidermal tissues.

Results: EM2 nanoemulsion was characterized by different techniques: Zeta Potential, Dynamic Light Scattering and Transmission Electron Microscopy. The formulation was tested for its antimicrobial activity (disk diffusion method and the dilution method) against 5 bacterial strains and 2 fungi. Levofloxacin and fluconazole were used as positive control. EM2 nanoemulsion (1 mg/mL) elicited an antifungal effect on Candida albicans and C. parapsilosis (inhibition areas were 29.6 and 30 mm, respectively). Similar effects were obtained for EM2 alone. The experiments performed on the 3D reconstructed human epidermal tissues were made in standard conditions that complied with OECD Test Guideline 439 and have ECVAM validation. The tissue models viability was not affected following samples application.

Conclusion: EM2 nanoemulsion produced an antifungal effect and did not display a significant skin irritative effect on the 3D-tissue model, thus the sample can serve as a promising formulation in skin care treatment

Conflicts of interest

The authors declare no conflict of interest.

This research was funded by the Project PN-III-P1 – 1.1-PD-2019 – 1231, no. 206/2020. (I. Z. M. P.)