Planta Med 2022; 88(15): 1551
DOI: 10.1055/s-0042-1759289
Poster Session II

Effect of Tormentillae tinctura on the barrier function of Caco-2 cells monolayer

Authors

  • A Kruk∗

    Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland

  • E Cymbor

    Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland

  • D Popowski

    Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland

  • J Piwowarski

    Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland

  • S Granica

    Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland
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Tormentillae rhizoma is a traditionally used pharmacopeial plant material with various applications related to gastrointestinal tract ailments. The plant material is taken orally, the most popular preparation is a tincture (Tormentillae tinctura, TT). Due to its tannin-reach composition, it can offer an opportunity for novel approaches in the therapy of Leaky Gut Syndrome [1], [2].

The research was aimed to establish the effect of TT on the gut barrier integrity with transepithelial permeability assessment of its constituents.

The cytotoxicity of the TT towards Caco-2 cells was investigated using the MTT test. The barrier functions of Caco-2 monolayer were examined by 24 h incubation with TT accompanied by real-time monitoring of the transepithelial electrical resistance (TEER). Analysis of substances permeating through the monolayer was conducted using UHPLC-DAD-MS.

It was shown that TT is not cytotoxic towards Caco-2 in a concentrations 0,0625 – 5 mg/ml. The addition of TT did not affect the condition of Caco-2 monolayer based on TEER monitoring. Significant differences for TT constituents with regard to their migration through monolayers were observed. Compounds with the best permeability were saponin aglycones, mainly tormentic acid derivatives, which were detected in the acceptor medium together with some triterpene glycosides. Instead, highly abundant in TT oligomeric procyanidins were not able to cross the monolayer.

The research has indicated, for the first time, which natural products contained in Tormentillae tincura can cross the intestinal barrier in vitro and potentially express their bioactivity beyond the gut lumen.

Project financial support: Polish National Science Centre research grant Sonatina 5 2021/40/C/NZ7/00 231.



Publication History

Article published online:
12 December 2022

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