Phenolics and polysaccharides isolated from the roots of Aconitum septentrionale, a Norwegian medicinal plant

Aconitum septentrionale Koelle (Ranunculaceae family) is a medicinal plant widely distributed in Norway, Sweden and Russia, and well known for its toxicity. Alkaloids, such as lappaconitine, lappaconine and septontrionin [1], [2], [3], as well as lipids and organic acids [4], [5] have previously been identified in the plant. The high content of alkaloids is responsible for the poisonous effects of the plant, which also limits its utilization. However, other types of bioactive natural products like phenolics and polysaccharides that are present in most Aconitum plants, have not been studied in A. septentrionale yet. The aim of this study was to explore the content of phenolics and polysaccharides in a water extract of this plant, and to study their immunomodulating effects.

The water extract was applied to a Diaion HP-20 absorbent column and eluted with water and methanol. The water fraction was purified by ion exchange chromatography and gel filtration to isolate the polysaccharides, while the 25 – 100% methanol fractions were subjected to C18 flash chromatography, Sephadex LH-20 and preparative HPLC. Several phenolic compounds and three types of polysaccharides were isolated from water extracts of A. septentrionale roots for the first time. Further structure analysis and bioactivity testing of the polysaccharides and phenolics are now under investigation and will be presented.

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• References

• 1 Ross SA, Pelletier SW, Aasen AJ. New norditetpenold alkaloids from Aconitum septetrionale. Tetrahedron 1992; 48: 1183-1192
• 2 Khairitdinova ED, Tsyrlina EM, Spirikhin LV. et al. Norditerpenoid alkaloids from Aconitum septentrionale K. Russian J Org Chem 2008; 44: 536-541
• 3 Goncharov AE, Politov AA, Pankrushina NA. et al. Isolation of lappaconitine from Aconitum septentrionale roots by adsorption. Chem Nat Comp 2006; 42: 336-339
• 4 Khomova TV, Gusakova SD, Glushenkova AI. Lipids of the roots of Aconitum septentrionale and of their processing wastes. Chem Nat Comp 1996; 32: 689-691
• 5 Jermstad A, Jensen KB. The constituents of Aconitum septentrionale. Pharm Acta Helv 1951; 26: 33-47

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Artikel online veröffentlicht:
12. Dezember 2022