Phytochemical profiles and Bioactivity of Nepeta phyllochlamys P. H. Davis

There are about 275 taxa of Nepeta L. in the world [1]. In Turkey, Nepeta includes 45 taxa, 18 of which are endemic [2]. Nepeta species are widely used in folk medicine because of their antispasmodic, diuretic, antiseptic, antitussive, antiasthmatic, and febrifuge activities [3]. Nepeta phyllochlamys P. H. Davis is a local endemic in Antalya province, Turkey. This study aimed to in vitro antioxidant and antimicrobial activity of extracts prepared with different solvents (n-hexane, ethyl alcohol 70%, and ethyl acetate) of N. phyllochlamys. The antioxidant activities of the extracts of N. phyllochlamys were determined by the DPPH• scavenging activity assay. Furthermore, the composition of hydrodistilled essential oil (yield 0.7%.) of N. phyllochlamys was determined by GC/GC-MS analysis. 70% ethanol extract of N. phyllochlamys showed higher antioxidant activity (IC₅₀: 0.32 ± 0.025 mg/ml). However, all extracts of the N. phyllochlamys demonstrated weaker antioxidant activity than the standard gallic acid.

According to the GC-MS analysis, the major components were determined as caryophyllene oxide (%18.2), β-pinene (%15.6) and linalool (%11.0).

N. phyllochlamys extracts were evaluated against four bacterial and five candidal reference strains. The extracts showed weak activity against all bacterial strains in the MIC range of 2000 – 8000 µg/mL. It was determined that the lowest MIC value (125 µg/mL) and the highest anticandidal effect were in n-hexane extract against C. tropicalis (ATCC750).

 

MissingLinkText

• References

• 1 http://www.worldfloraonline.org
• 2 Güner A, Aslan S, Ekim T, Vural M, Baba MT. (eds) Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul: Nezahat Gökyiğit Botanic Garden and Flora Research Association Publications; 2012: 564-568
• 3 Tepe B, Daferera D, Tepe AS. et al. Antioxidant activity of the essential oil and various extracts of Nepeta flavida Hub. -Mor. from Turkey. Food Chemistry 2007; 103: 1358-1364

Publication History

Article published online:
12 December 2022