Antiosteoclastogenic active compounds from the roots of Broussonetia kazonoki

To obtain antiosteoclastogenic active compounds from the natural sources, 19 compounds were isolated from the roots of Broussonetia kazinoki Siebold. We also report the isolation and structure determination of broussonol I from a natural source. The chemical structure of the isolated compounds was determined using conventional NMR and MASS data. Absolute configurations were assigned using time-dependent density functional theory calculations and Electronic Circular Dichroism (ECD) spectroscopy. The isolated 19 compounds ([Fig. 1]) were screened for their effects on RANKL-induced osteoclast formation using RAW264.7 cells. Of them, broussonols F, G, and K showed dose-dependent antiosteoclastogenic activities with IC₅₀ value of 6.33 – 14.13 µM against BMMs cells. Broussonol K exhibited the most potent inhibitory activity for osteoclast formation and possessed bone resorption suppressive activity.

Fig. 1 

• References

• 1 Vu NK, Ha MT, Kim CS. et al. Structural characterization of prenylated compounds from Broussonetia kazinoki and their antiosteoclastogenic activity. Phytochemistry 2021; 188: 112791
• 2 Tran PT, Ngo TQM, Lee S. et al. Identification of anti-osteoclastogenic compounds from Cleistocalyx operculatus flower buds and their effects on RANKL-induced osteoclastogenesis. J Funct Foods 2019; 60: 103388

Publication History

Article published online:
12 December 2022