In vitro cultures of antimicrobial and antibiofilm isoquinoline alkaloids containing ornamental plant Corydalis cheilanthifolia Hemsl

Corydalis cheilanthifolia Hemsl. is an ornamental perennial of Papaveraceae originating from East Asia and commonly planted in hobby and public gardens. Our previous studies demonstrated high content of several isoquinoline alkaloids and polyphenolic compounds in various parts of cultivated C. cheilanthifolia [1].

In this study, we established in vitro culture of this species in order to provide a model for manipulation of alkaloid and polyphenol composition towards optimal antimicrobial properties and discovery of various factors influencing metabolic profile. Also, a method for fast clonal propagation may facilitate selection of superior quality plant material for pharmaceutical use.

In vitro cultures initiated from seedlings were established in form of proliferating microshoots, callus tissue and cell suspension. Out of several tested media, the MH3 composition [2] supplemented with benzyladenine and indoleacetic acid were optimal for growth and viability optimization experiments. The presence of targeted alkaloids as well as phenolic compounds was confirmed by chromatographic analysis. The extracts were found effective against human pathogens such as S. aureus, P. aeruginosa, C. albicans and multidrug resistant Helicobacter pylori [1], [3]. Further investigations to reveal the relationships between the antimicrobial properties of individual constituents and the composition and quantities of alkaloids and polyphenols in cell and tissue cultures of this species. In conclusion, a significant antimicrobial potential of C. cheilantifolia and the possibility of obtaining the active compounds from in vitro cultures provide a feasible model for optimization of phytochemical composition targeting specific pathogens.

Funding

National Science Centre of Poland (NCN) Sonata 15, grant 2019/35/D/NZ7/00 266.

• References

• 1 Zielinska S, Dziągwa-Becker M, Junka A. et al. Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents. Molecules 2021; 26: 4778
• 2 Fulcheri C, Morard P, Henry M. Stimulation of the growth and the triterpenoid saponin accumulation of Saponaria officinalis cell and Gypsophila paniculata root suspension cultures by improvement of the mineral composition of the media. J Agric Food Chem 1998; 46: 2055-2061
• 3 Krzyżek P, Junka A, Słupski W. et al. Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori. Pathogens 2021; 10: 1033

Publication History

Article published online:
12 December 2022