Quantification of Camellia oleifera extract in complete feed with UHPLC-MS/MS using a double “one-point” standard addition

A specific and sensitive method for the quantification of Camellia oleifera in complete feed based on UHPLC-MS/MS has been developed and validated. The additive studied is a natural commercial feed additive (Cosap^®, Nor-Feed, France) used in animal nutrition, consisting of tea seed extract in which camelliaside A has been identified and quantified. Camelliaside A was used as phytomarker for the quantification of the additive with a level of 3 µg/g of phytomarker in complete feed. To compensate for the loss of camelliaside A during the extraction step, a glycosylated flavanone is used as internal standard. Because of matrix effects that can occur in mass spectrometry, standard addition method was selected for the quantification. To decrease the workload of the standard addition method; a simplified approach based on the “one-point” addition was used for camelliaside A and internal standard permitting the utilization of the method in routine. The method was developed and validated in-house in accordance with the guidelines recommended by IUPAC [1] for the quantification of Camellia oleifera extract in complete feed. This method of analysis has all the prerequisites to be used by European authorities as part of the registration of a feed additive [2].

• References

• 1 Thompson M, Ellison SLR, Wood R. . Pure and Applied Chemistry 2002; 74: 835-855
• 2 FEEDAP. . EFSA Journal 2012; 10: 2536

Publication History

Article published online:
12 December 2022