Chemodiversity and cosmetic potential of Dendrobium fimbriatum (Orchidaceae) fungal community

Inhibition of tyrosinase, a key enzyme in melanin biosynthesis, is of great interest for the cosmetic industry, due to its skin-whitening properties and skin hyperpigmentation or age spots reduction effects [1].

Fungal melanin has important ecological and biochemical functions for persistence in the environment and within organisms [2]. Recently, fungal endophyte have shown anti-tyrosinase activities highlighting plant fungal community as an underexploited source of promising inhibitors [3].

The cultivable part of the fungal community of Dendrobium fimbriatum Hooke, 25 species belonging predominantly to Fusarium, Trichoderma, Colletotrichum, Curvularia, and Phomopsis genus, was evaluated as a source of cosmetic active ingredients.

The metabolite profiles of all strains were investigated by a dereplication strategy based on LC-HRMS/MS and molecular network (MN) representation based on fragmentation similarities. In parallel, extracts were screened on enzymatic bioautography methods for anti-tyrosinase and antioxidant activities. Active compounds were desorbed and analyzed by MS for annotation using MS/MS databases with taxonomical information [4], [5].

The chemodiversity of the community was significant with terpenoids, amino acids and peptides, alkaloids, fatty acids, and polyketides compounds. MN representation revealed similitude/difference in metabolic pattern of the extracts that were used for making correlation with the bioassay.

All extracts had anti-tyrosinase and antioxidant actions with some similar and species or genus specific active compounds. The resulting MN highlighted various analogues active compounds.

The fungal community of Dendrobium fimbriatum appears as a rich source of cosmetic active ingredients. Indeed, the lack of annotation for several clusters indicate potential novel tyrosinase inhibitors.

The authors declare no conflict of interest.

• References

• 1 Zaid AN, Al Ramahi R. Depigmentation and anti-aging treatment by natural molecules. Curr Pharm Des 2019; 25: 2292-2312
• 2 Smith DFQ, Casadevall A. The role of melanin in fungal pathogenesis for animal hosts. In: Rodrigues ML. Hrsg. Fungal Physiology and Immunopathogenesis. Cham: Springer International Publishing; 2019: 1-30
• 3 Fernandes MS, Kerkar S. Microorganisms as a source of tyrosinase inhibitors: A review. Ann Microbiol 2017; 67: 343-358
• 4 Rutz A, Dounoue-Kubo M, Ollivier S. et al. Taxonomically informed scoring enhances confidence in natural products annotation. Front Plant Sci 2019; 10: 1329
• 5 Allard P-M, Genta-Jouve G, Wolfender J-L. Deep metabolome annotation in natural products research: Towards a virtuous cycle in metabolite identification. Curr Opin Chem Biol 2017; 36: 40-49

Publication History

Article published online:
12 December 2022