An Improved Bioavailability of Total Lutein Oxidized Products (LOPs) Extracted from Tagetes erecta Flower Petals in C57BL/6 Mice

Correspondence to: [e-mail]

Background: The lutein extracted from shade dried Tagetes erecta flower petal powder by simultaneous solvent extraction and saponification, was exposed to sunlight (31 ± 2°C) for 10 days. The resultant lutein oxidized products (LOP) exhibited significant inhibition of pancreatic lipase in in-vitro assay with IC₅₀ of 1.5953 μg/mL.

Materials and Methods: Bioavailability and absorption kinetics of LOPs in comparison with lutein (parent molecule) were analyzed on C57BL/6J mice. Time course plasma kinetics was studied by collecting blood plasma, liver, intestine and eyes after first, second, third, sixth, and ninth hours of intubation.

Results: Postprandial LOPs concentration in plasma was maximum at second hour. The area under concentration (AUC) and area under moment concentration (AUMC) of LOPs were 1,139.418 and 17,750.69 pg/h²/mL, respectively, with a half-life of 11.353 hours. The mean residence time (MRT) of LOPs was 15.573 hours with a volume of distribution (Vd) of 2.874 and clearance (CL) of 0.175. The eyes had 59.80 pg/mL at ninth hour after pooling due to less concentration of LOPs in single eye. Whereas, mean lutein concentration in plasma, liver, intestine, and eye was significantly less in lutein in comparison with LOPs.

Conclusion: Thus the above data suggest that LOPs reach systemic circulation and target tissue unaltered and enhance the absorption rate in comparison to the parental compound lutein and with further in vivo examinations, LOPs can be developed as a potent nutraceutical with antiobesity action.

Die Autoren geben an, dass kein Interessenkonflikt besteht.

Publikationsverlauf

Artikel online veröffentlicht:
22. August 2022