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DOI: 10.1055/s-0042-1746493
A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature
Introduction Microvascular dysfunction is a fundamental process in the pathogenesis of salivary gland diseases. Hence, preserving and restoring the integrity of the microcirculation might represent a promising therapeutic strategy for these pathologies. The underlying mechanisms, however, remain poorly understood, partly due to the unavailability of adequate +in vivo+ models.
Methods Employing different technical approaches such as multiphoton +in vivo+ microscopy, confocal microscopy, and multi-channel flow cytometry, we analyzed the trafficking and interactive behavior of different immune cell populations as well as the permeability in the salivary gland microvasculature of mice.
Results Superfusion of submandibular glands with a tumor necrosis factor containing solution induced the expression of distinct signaling and adhesion molecules on microvascular endothelial cells, thus promoting intravascular interactions of neutrophils and classical monocytes as well as their subsequent infiltration of the perivascular tissue. These processes were associated with an enhanced microvascular permeability.
Conclusions We established a novel experimental in vivo approach that enables us to examine essential microvascular processes in the salivary gland on a cellular and molecular level. This model allows us to identify specific pathogenetic factors as novel therapeutic targets in salivary gland disorders.
die Deutsche Forschungsgemeinschaft (SFB 914).
Conflict of Interest
The author declares that there is no conflict of interest.
Publication History
Article published online:
24 May 2022
© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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