Nuklearmedizin 2022; 61(02): 168-169
DOI: 10.1055/s-0042-1746033
Abstracts | NuklearMedizin 2022
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Evaluation of different vascular access ways in an extracorporeal circulation model for quantitative determination of dual PET/MRI arterial input functions in mice

F. Gierse
1   University of Münster, European Institute for Molecular Imaging, Münster
,
J. Cufe
2   University Hospital Münster, Department of Nuclear Medicine, Münster
,
B. Maus
3   University of Münster, Translational Research Imaging Center, Münster
,
K. Schäfers
1   University of Münster, European Institute for Molecular Imaging, Münster
,
S. Hermann
1   University of Münster, European Institute for Molecular Imaging, Münster
,
M. Claesener
2   University Hospital Münster, Department of Nuclear Medicine, Münster
,
C. Faber
3   University of Münster, Translational Research Imaging Center, Münster
,
M. Schäfers
2   University Hospital Münster, Department of Nuclear Medicine, Münster
,
F. Büther
2   University Hospital Münster, Department of Nuclear Medicine, Münster
,
P. Backhaus
2   University Hospital Münster, Department of Nuclear Medicine, Münster
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Ziel/Aim Image-derived measurements of the dynamic arterial blood concentration (arterial input function, AIF) for pharmacokinetic modeling (PKM) are challenging for both PET and MRI in mice. Alternatively, an extracorporeal circulation approach can be used [1]. We evaluated the quantitative precision of extracorporeally measured AIFs of MRI contrast agent (CA) and its radioactive analog by dual recordings and compared dispersion effects in two different vascular access ways.

Methodik/Methods 13 mice were measured in a 9.4 T Bruker Biospec small animal MRI. 100µl of 35mM Gd-DO3A-butrol (Gadovist) mixed with its radioactive analog Ga-68-DO3A-butrol (5.7 Mbq±3.0) were injected. An extracorporeal circulation was applied either from the femoral artery to tail vein (n=8) or from the carotid bifurcation to the jugular vein (n=5). The circulations featured MR measurement reservoirs with a volumetric distance of 14.1 µl from the femoral artery and a negligible distance from the carotid artery (1.6µl). Further downstream, blood radioactivity was assessed in a MR compatible measuring unit (Swisstrace, Twilite). A Golden-angle Radial Sparse Parallel (GRASP) sequence, combined with a compressed sensing MP2RAGE sequence for T1 mapping, was used for DCE-MRI.

Ergebnisse/Results Good quantitative correspondence on the whole range of dynamic CA and radioactive analogs was observed. Dispersion effects in the femoral artery shunt were corrected using kernels based on single gamma variate functions, resulting in AIFs resembling the carotid shunt where dispersion seemed negligible.

Schlussfolgerungen/Conclusions Correspondence between PET and MRI quantification of extracorporeal AIFs and agreement of the two different shunt approaches after dispersion correction indicate a high potential of our setup to set the basis for precise dual PET/MRI PKM in mice.


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Artikel online veröffentlicht:
14. April 2022

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