Planta Med 2017; 83(07): 624-630
DOI: 10.1055/s-0042-119950
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Relaxant Effect of Sodium Tanshinone IIA Sulphonate on Mouse Tracheal Smooth Muscle

Yi-Lin Zhang
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Jia-Wen Xu
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Xiong-Hao Wu
2   Department of Physiology, School of Basic Science, Guangzhou Medical University, Guangzhou, China
,
Peng-Xiao Chen
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Fang Wei
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Dong-Dong Gao
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Xiao-Wen Chen
3   Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
,
Yu-Li Luo
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Yun-Xin Zhu
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Jiehong Huang
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
,
Ya-Xia Tan
3   Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
,
Lei Zhao
2   Department of Physiology, School of Basic Science, Guangzhou Medical University, Guangzhou, China
,
Wen-Liang Zhou
1   School of Life Sciences, Sun Yat-sen University, Guangzhou, China
› Author Affiliations
Further Information

Publication History

received 04 August 2016
revised 14 October 2016

accepted 24 October 2016

Publication Date:
09 December 2016 (online)

Abstract

Sodium tanshinone IIA sulphonate, a water-soluble derivative of tanshinone IIA, has been proven to possess versatile biological properties, but its pharmacological effect on tracheal smooth muscle remains elusive. This paper presents a study on the relaxant effect and underlying mechanisms of sodium tanshinone IIA sulphonate on mouse tracheal smooth muscle. The relaxant effect of sodium tanshinone IIA sulphonate was evaluated in mouse tracheal rings using a mechanical recording system. Intracellular Ca2+ concentration was measured in primary cultured tracheal smooth muscle cells using confocal imaging system. The results showed that sodium tanshinone IIA sulphonate induced dose-dependent relaxation of mouse tracheal rings in a β-adrenoceptor- and epithelium-independent manner. Pretreatment with the ATP-sensitive K+ channel blocker glibenclamide partly attenuated the relaxation response. Administration of sodium tanshinone IIA sulphonate notably inhibited the extracellular Ca2+-induced contraction. High KCl or carbachol-evoked elevation in the intracellular Ca2+ concentration was also abrogated by sodium tanshinone IIA sulphonate in tracheal smooth muscle cells. In conclusion, the tracheal relaxant effect of sodium tanshinone IIA sulphonate was independent of β-adrenoceptor and airway epithelium, mediated primarily by inhibition of extracellular Ca2+ influx via L-type voltage-dependent Ca2+ channels and partially by activation of the ATP-sensitive K+ channel. These results indicate the potential therapeutic value of sodium tanshinone IIA sulphonate for asthma treatment.

 
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