Amyrins from Protium heptaphyllum Reduce High-Fat Diet-Induced Obesity in Mice via Modulation of Enzymatic, Hormonal And Inflammatory Responses

Karine Maria Martins Bezerra Carvalho; Tiago Sousa de Melo; Karina Moura de Melo; Ana Luiza Gomes Quinderé; Francisca Tuelly Bandeira de Oliveira; Ana Flávia Seraine Custódio Viana; Paulo Iury Gomes Nunes; Josiane da Silva Quetz; Daniel de Araújo Viana; Armenio André de Carvalho Almeida da Silva; Alexandre Havt; Said Gonçalves da Cruz Fonseca; Mariana Helena Chaves; Vietla Satyanarayana Rao; Flávia Almeida Santos

doi:10.1055/s-0042-114222

Planta Medica, Table of Contents

Planta Med 2017; 83(03/04): 285-291
DOI: 10.1055/s-0042-114222

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Amyrins from Protium heptaphyllum Reduce High-Fat Diet-Induced Obesity in Mice via Modulation of Enzymatic, Hormonal And Inflammatory Responses

Karine Maria Martins Bezerra Carvalho

¹Post-Graduate Program in Medical Sciences, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Tiago Sousa de Melo

³Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Karina Moura de Melo

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Ana Luiza Gomes Quinderé

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Francisca Tuelly Bandeira de Oliveira

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Ana Flávia Seraine Custódio Viana

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Paulo Iury Gomes Nunes

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Josiane da Silva Quetz

⁴Haroldo Juaçaba Hospital, Cancer Institute of Ceará, Fortaleza, Ceará, Brazil

,

Daniel de Araújo Viana

⁵Pathology and Legal Medicine Laboratory, Faculty of Veterinary Science, State University of Ceará, Fortaleza, Ceará, Brazil

,

Armenio André de Carvalho Almeida da Silva

⁶Department of Organic Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil

,

Alexandre Havt

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Said Gonçalves da Cruz Fonseca

³Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Mariana Helena Chaves

⁶Department of Organic Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil

,

Vietla Satyanarayana Rao

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

,

Flávia Almeida Santos

²Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil

› Author Affiliations

Abstract

Obesity remains a global problem. In search of phytochemicals that have antiobesity potential, this study evaluated α,β-amyrin, a triterpenoid mixture from Protium heptaphyllum, on high-fat diet-induced obesity in mice. Groups of mice (n = 8) were fed a normal diet or a high-fat diet, and were orally treated or not treated with either α,β-amyrin (10 or 20 mg/kg) or sibutramine (10 mg/kg) for 15 weeks. Variables measured at termination were body weight, visceral fat accumulation, adipocyte surface area, peroxisome proliferator-activated receptor gamma, and lipoprotein lipase expressions in adipose tissue, the levels of plasma glucose and insulin, the satiety hormones ghrelin and leptin, the digestive enzymes amylase and lipase, and the inflammatory mediators TNF-α, interleukin-6, and MCP-1. Results showed that α,β-amyrin treatment resulted in lower high-fat diet-induced increases in body weight, visceral fat content, adipocyte surface area, peroxisome proliferator-activated receptor gamma, and lipoprotein lipase expressions, and blood glucose and insulin levels. Additionally, the markedly elevated leptin and decreased ghrelin levels seen in the high-fat diet-fed control mice were significantly modulated by α,β-amyrin treatment. Furthermore, α,β-amyrin decreased serum TNF-α and MCP-1. These results suggest that α,β-amyrin could be beneficial in reducing high-fat diet-induced obesity and associated disorders via modulation of enzymatic, hormonal, and inflammatory responses.

Key words

Protium heptaphyllum - Burseraceae - α,β-amyrin - pentacyclic triterpene - high-fat diet - antiobesity

Full Text

References

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