Metabolic Inflexibility with Obesity and the Effects of Fenofibrate on Skeletal Muscle Fatty Acid Oxidation

Kristen E. Boyle; Jacob E. Friedman; Rachel C. Janssen; Chantal Underkofler; Joseph A. Houmard; Neda Rasouli

doi:10.1055/s-0042-111517

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2017; 49(01): 50-57
DOI: 10.1055/s-0042-111517

Endocrine Research

Metabolic Inflexibility with Obesity and the Effects of Fenofibrate on Skeletal Muscle Fatty Acid Oxidation

Authors

Kristen E. Boyle

¹Division of Nutrition, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA
Jacob E. Friedman

²Division of Neonatology, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA
Rachel C. Janssen

²Division of Neonatology, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA
Chantal Underkofler

³Division of Endocrinology, Metabolism and Diabetes, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA
Joseph A. Houmard

⁴Department of Kinesiology, East Carolina University, Greenville, NC, USA
Neda Rasouli

³Division of Endocrinology, Metabolism and Diabetes, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA

⁵Veterans Administration Eastern Colorado Health Care System, Denver, CO, USA

Abstract

This study was designed to investigate mechanisms of lipid metabolic inflexibility in human obesity and the ability of fenofibrate (FENO) to increase skeletal muscle fatty acid oxidation (FAO) in primary human skeletal muscle cell cultures (HSkMC) exhibiting metabolic inflexibility. HSkMC from 10 lean and 10 obese, insulin resistant subjects were treated with excess fatty acid for 24 h (24hFA) to gauge lipid-related metabolic flexibility. Metabolically inflexible HSkMC from obese individuals were then treated with 24hFA in combination with FENO to determine effectiveness for increasing FAO. Mitochondrial enzyme activity and FAO were measured in skeletal muscle from subjects with prediabetes (n=11) before and after 10 weeks of fenofibrate in vivo. 24hFA increased FAO to a greater extent in HSkMC from lean versus obese subjects (+49% vs. +9%, for lean vs. obese, respectively; p<0.05) indicating metabolic inflexibility with obesity. Metabolic inflexibility was not observed for measures of cellular respiration in permeabilized cells using carbohydrate substrate. Fenofibrate co-incubation with 24hFA, increased FAO in a subset of HSkMC from metabolically inflexible, obese subjects (p<0.05), which was eliminated by PPARα antagonist. In vivo, fenofibrate treatment increased skeletal muscle FAO in a subset of subjects with prediabetes but did not affect gene transcription or mitochondrial enzyme activity. Lipid metabolic inflexibility observed in HSkMC from obese subjects is not due to differences in electron transport flux, but rather upstream decrements in lipid metabolism. Fenofibrate increases the capacity for FAO in human skeletal muscle cells, though its role in skeletal muscle metabolism in vivo remains unclear.

Key words

mitochondria - fat oxidation - insulin resistance

Full Text

References

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