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Drug Res (Stuttg) 2016; 66(10): 527-531
DOI: 10.1055/s-0042-111008
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Anti-Respiratory Syncytial Virus Compounds from Two Endophytic Fungi Isolated from Nigerian Medicinal Plants

P. F. Uzor
1   Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka, Nigeria
,
D. C. Odimegwu
2   Division of Pharmaceutical Microbiology and Biotechnology, Department of Pharmaceutics, University of Nigeria, Nsukka, Nigeria
,
W. Ebrahim
3   Heinrich-Heine-Universität, Institut für Pharmazeutische Biologie und Biotechnologie, Düsseldorf, Germany
4   Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt
,
P. O. Osadebe
1   Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka, Nigeria
,
N. J. Nwodo
1   Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka, Nigeria
,
F. B. C. Okoye
5   Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria
,
Z. Liu
3   Heinrich-Heine-Universität, Institut für Pharmazeutische Biologie und Biotechnologie, Düsseldorf, Germany
,
P. Proksch
3   Heinrich-Heine-Universität, Institut für Pharmazeutische Biologie und Biotechnologie, Düsseldorf, Germany
› Author Affiliations
Further Information

Publication History

received 28 March 2016

accepted 22 June 2016

Publication Date:
27 July 2016 (online)

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Abstract

Background: Respiratory syncytial virus (RSV) is known to cause severe respiratory infections particularly in infants younger than 2 years of age. The only approved drug, ribavirin, is expensive and is not likely to improve therapeutic outcome, thereby necessitating the search for safer and more potent alternatives from natural sources such as endophytic fungi. The present study aimed to investigate the anti-RSV activity of compounds from endophytic fungi.

Methods: Two endophytic fungi Colletotrichum gloeosporioides and Pestalotiopsis thea were isolated from the fresh leaves of the host Nigerian plants Anthocleista djalonensis and Fagara zanthoxyloides, respectively. After fermentation in solid rice media, C. gloeosporioides afforded 4 known compounds 4-hydroxybenzoic acid (1), vanillic acid (2), ferulic acid (3) and Nb-acetyltryptamine (4) while P. thea afforded 3 known compounds chloroisosulochrin (5), ficipyrone A (6) and pestheic acid (7). The compounds were investigated for their anti-RSV activity using the HEP-2 cell lines and ribavirin as the standard drug.

Results: Compound 5 was found to show the strongest inhibition of the RSV with IC50 of 4.22±1.03 µM (ribavirin 4.91±1.85 µM). Other compounds showed moderate inhibition of the virus (IC50 ranging from 45.00±0.98 to 259.23±2.36 µM).

Conclusion: The results of the present study have shown that chloroisosulochrin (5), isolated from an endophytic fungus P. thea, possesses strong activity against RSV.

Key words

chloroisosulochrin - colletotrichum gloeosporioides - endophytic fungi - ficipyrone A - pestalotiopsis thea - pestheic acid - respiratory syncytial virus
 

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