Planta Med 2016; 82(14): 1295-1301
DOI: 10.1055/s-0042-104659
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Characterization of the Volatile and Nonvolatile Fractions of Heartwood Aqueous Extract from Pterocarpus marsupium and Evaluation of Its Cytotoxicity against Cancer Cell Lines

Fabio Gosetti
1   University of Piemonte Orientale, Department of Science and Technological Innovation, Alessandria, Italy
,
Ugo Chiuminatto
2   AB Sciex, Brugherio (MB), Italy
,
Simona Martinotti
1   University of Piemonte Orientale, Department of Science and Technological Innovation, Alessandria, Italy
,
Bianca Bolfi
1   University of Piemonte Orientale, Department of Science and Technological Innovation, Alessandria, Italy
,
Elia Ranzato
1   University of Piemonte Orientale, Department of Science and Technological Innovation, Alessandria, Italy
,
Marcello Manfredi
1   University of Piemonte Orientale, Department of Science and Technological Innovation, Alessandria, Italy
3   ISALIT S. r. l., Novara, Italy
,
Emilio Marengo
1   University of Piemonte Orientale, Department of Science and Technological Innovation, Alessandria, Italy
› Institutsangaben
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Publikationsverlauf

received 26. November 2015
revised 25. Februar 2016

accepted 02. März 2016

Publikationsdatum:
28. April 2016 (online)

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Abstract

Pterocarpus marsupium is a well-known plant due to its healing properties, in particular, the use of its aqueous extract is able to reduce blood sugar levels and blood triglyceride concentrations. Although this plant has already been widely studied, a complete characterization of its aqueous extract has not been reported. The present study deals with the characterization of the aqueous extract of P. marsupium in order to obtain a full fingerprint of the volatile and nonvolatile constituents. The volatile constituents were identified by CG-MS, whereas the nonvolatile fraction was characterized by UHPLC-MS/MS using a nontarget approach. Several compounds were identified, in particular, polyphenolic species belonging to the class of proanthocyanidins. Cytotoxicity tests were carried out on four different cancer cell lines and three different non-tumoral cell lines. Preliminary results indicate a selective cytotoxicity of the aqueous extract towards the cancer cells. The potential cytotoxicity due to the presence of metals in the aqueous extract was ruled out by testing an aqueous mixture of the metals at the same concentration found in the P. marsupium extract.

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